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Heterogeneity of microtubules in dividing sea urchin eggs revealed by immunofluorescence microscopy: Spindle microtubules are composed of tubulin isotypes different from those of astral microtubules

1990; Wiley; Volume: 16; Issue: 4 Linguagem: Inglês

10.1002/cm.970160404

1097-0169

Mikako Oka, Takao Arai, Yukihisa Hamaguchi,

Photosynthetic Processes and Mechanisms

Abstract The heterogeneity of mitotic microtubules in dividing sea urchin eggs was investigated by indirect immunofluorescence using five anti‐α‐tubulin (YL1/2, DM1A, E3B8, D2D6, and 6‐11B‐1) and two anti‐β‐tubulin (E6B6 and DM1B) antibodies. These antibodies were divided into four classes in regard to the different immunofluorescent staining patterns: class I, which strongly stained both the spindle and aster (YL1/2, DM1A, E3B8 and E6B6); class II, which strongly stained the spindle but weakly stained the aster (D2D6); class III, which stained only the aster (DM1B); and class IV, which did not stain the mitotic apparatus (6‐11B‐1). These results suggest that tubulin isotypes are distributed differently in the sea urchin mitotic microtubules and that α‐tubulin isotype(s) recognized by D2D6 is (are) localized mainly in spindle microtubules, whereas β‐tubulin isotype(s) recognized by DM1B is (are) found only in astral microtubules.