Sequence conservation in the α and β subunits of pyruvate dehydrogenase and its similarity to branched‐chain α‐keto acid dehydrogenase
1991; Wiley; Volume: 282; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(91)80479-m
ISSN1873-3468
AutoresIsaiah D. Wexler, S. Hemalatha, Mulchand S. Patel,
Tópico(s)Diet and metabolism studies
ResumoAmino acid sequence comparison of 8α and 6β subunits of the α‐keto acid dehydrogenase (E 1 ) component of the pyruvate dehydrogenase complex and branched‐chain α‐keto acid dehydrogenase complex from multiple species was performed by computer analysis. In addition to 2 previously recognized regions of homology in the α subunit, a 3rd region of extensive homology was identified in E 1 α, and may be one of the sites involved in subunit interaction. E 1 β contains 4 regions of extensive homology. Region 1 contains 10 amino acids that are homologous to a 10‐amino acid stretch in Escherichia coli E 1 . Regions 2 and 3 have sequence homologies with other dehydrogenases suggesting that these regions may be involved in catalysis.
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