Produção Nacional
Revisado por pares
Ultra-Deep Sequencing Reveals the microRNA Expression Pattern of the Human Stomach
2010; Public Library of Science; Volume: 5; Issue: 10 Linguagem: Inglês
10.1371/journal.pone.0013205
ISSN1932-6203
AutoresÂndrea Ribeiro‐dos‐Santos, André Salim Khayat, Artur Silva, Dayse O. Alencar, Jessé Lobato, Larissa Luz, Daniel Guariz Pinheiro, Leonardo Varuzza, Mônica Assumpção, Paulo Pimentel de Assumpção, Sidney Emanuel Batista dos Santos, Dalila Lucíola Zanette, Wilson A. Silva, Rommel Mário Rodríguez Burbano, Sylvain Darnet,
Tópico(s)RNA modifications and cancer
ResumoBackground While microRNAs (miRNAs) play important roles in tissue differentiation and in maintaining basal physiology, little is known about the miRNA expression levels in stomach tissue. Alterations in the miRNA profile can lead to cell deregulation, which can induce neoplasia. Methodology/Principal Findings A small RNA library of stomach tissue was sequenced using high-throughput SOLiD sequencing technology. We obtained 261,274 quality reads with perfect matches to the human miRnome, and 42% of known miRNAs were identified. Digital Gene Expression profiling (DGE) was performed based on read abundance and showed that fifteen miRNAs were highly expressed in gastric tissue. Subsequently, the expression of these miRNAs was validated in 10 healthy individuals by RT-PCR showed a significant correlation of 83.97% (P<0.05). Six miRNAs showed a low variable pattern of expression (miR-29b, miR-29c, miR-19b, miR-31, miR-148a, miR-451) and could be considered part of the expression pattern of the healthy gastric tissue. Conclusions/Significance This study aimed to validate normal miRNA profiles of human gastric tissue to establish a reference profile for healthy individuals. Determining the regulatory processes acting in the stomach will be important in the fight against gastric cancer, which is the second-leading cause of cancer mortality worldwide.
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