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Agrobacterium rhizogenes Transformation of the Phaseolus spp.: A Tool for Functional Genomics

2006; American Phytopathological Society; Volume: 19; Issue: 12 Linguagem: Inglês

10.1094/mpmi-19-1385

1943-7706

Georgina Estrada‐Navarrete, Xóchitl Alvarado-Affantranger, Juan-Elías Olivares, Claudia Díaz-Camino, Olivia Santana, Enrique G. Murillo, Gabriel Guillén, Nayeli Sánchez-Guevara, Jorge Acosta-Dibarrat, Carmen Quinto, Dongxue Li, Peter M. Gresshoff, Federico Sánchez,

Plant nutrient uptake and metabolism

A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, landraces, and cultivars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. Induced hairy roots are robust and grow quickly. The transformation frequency is between 75 and 90% based on the 35-S promoter-driven green fluorescent protein and β-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal determinate nodules that fix nitrogen as efficiently as inoculated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiology, root metabolism, and root–microbe interactions.