Artigo Acesso aberto Produção Nacional Revisado por pares

Occurrence of Ralstonia solanacearum on olive tree in Brazil

2014; Grupo Paulista de Fitopatologia; Volume: 40; Issue: 2 Linguagem: Inglês

10.1590/0100-5405/1983

ISSN

1983-2729

Autores

Nilvanira Donizete Tebaldi, L. Nunes Leite, Janaína Marianno de Marque, Mayara Cristina Aliandro Furlanetto, Lara Caroline Borges Moreira Mota,

Tópico(s)

Banana Cultivation and Research

Resumo

In 2011, olive trees (Olea europaea L.) in a field in Camanducaia, Minas Gerais State, Brazil, showed symptoms of permanent leaf wilting, stem rot, phelloderm loss near the ground and vascular tissue discoloration (Figure 1).Bacterial exudation from infected tissue was observed under the microscope; then, the pathogen was isolated on nutrient-agar.Bacterial identification was done and the following characteristics were evidenced: irregular colonies that were white, smooth, flowing, opaque on 523-medium after 2-3 days at 28 °C, including dissemination of brown pigment, and Gramnegative; oxidation/fermentation: strictly aerobic, colonies showing reddish center and white edges on Kelman-medium, oxidasepositive, catalase-positive, biovar 1 (maltose, lactose, cellobiose, mannitol, sorbitol and dulcitol-negative), growth at 37 °C, gelatin liquefaction-negative, starch hydrolysis-negative, esculinnegative, arginine-positive, motility-positive and hypersensitivity

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