Sulfonyl Fluoride Analogues as Activity‐Based Probes for Serine Proteases
2012; Wiley; Volume: 13; Issue: 16 Linguagem: Inglês
10.1002/cbic.201200531
ISSN1439-7633
AutoresDavid A. Shannon, Christian Gu, Christopher J. McLaughlin, Markus Kaiser, Renier A. L. van der Hoorn, Eranthie Weerapana,
Tópico(s)Chemical Synthesis and Analysis
ResumoEnriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.
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