Photochemical cleavage of single- and double-stranded oligonucleotides by 3-(p-tolylamino)-1,5-azulenequinone
2001; Elsevier BV; Volume: 42; Issue: 33 Linguagem: Inglês
10.1016/s0040-4039(01)00963-7
ISSN1873-3581
AutoresFu‐Yuan Tsai, Shwu‐Bin Lin, Shwu‐Chen Tsay, Wei-Chen Lin, Chia-Lin Hsieh, Shih Hsien Chuang, Lou‐Sing Kan, Jih Ru Hwu,
Tópico(s)Photochromic and Fluorescence Chemistry
ResumoIrradiation, with 350 nm UV light, of specially designed and synthesized single- and double-stranded oligodeoxyribonucleotides in the presence of 3-(p-tolylamino)-1,5-azulenequinone produced fragments resulting from the cleavage at the deoxyguanosine residue only. The cleaving efficiency was greater for a single strand than a double helix. The efficiency was increased for a less stable double helix, or while the deoxyguanosine residue therein was located at a bulge, at a hairpin loop, or towards the end of the helix.
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