The suitability of using cryopreservation of spermatozoa for the conservation of genetic diversity in African catfish (Clarias gariepinus)
1993; Elsevier BV; Volume: 106; Issue: 2 Linguagem: Inglês
10.1016/0300-9629(93)90519-a
ISSN0300-9629
AutoresL.D. Van Der Walt, F.H. van der Bank, G.J. Steyn,
Tópico(s)Sperm and Testicular Function
Resumo1. The effect of cryopreservation of semen on expected Hardy-Weinberg proportions in the f1 progeny of selected breeding pairs was evaluated. 2. Four different African catfish breeding pairs were selected, each pair displaying different heterozygous alleles at the glucose-6-phosphate isomerase-1 or 2 loci. 3. Equal volumes of ova from each female were artificially inseminated with cryopreserved or fresh semen obtained from males possessing corresponding genotypes. 4. A comparison of growth rates between f1 groups of offspring produced from fresh and cryopreserved semen was made. 5. The G-test for goodness of fit showed no significant differences from expected Hardy-Weinberg proportions for allele frequencies obtained in the f1 progeny. 6. The application of cryopreservation for the conservation of genetic diversity is discussed.
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