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Plasmodium falciparum: Induction of resistance to mefloquine in cloned strains by continuous drug exposure in vitro

1988; Elsevier BV; Volume: 67; Issue: 2 Linguagem: Inglês

10.1016/0014-4894(88)90082-3

1090-2449

A.M.J. Oduola, Wilbur K. Milhous, Norman F. Weatherly, Jean H. Bowdre, R. E. Desjardins,

Antibiotic Resistance in Bacteria

A genetically homogeneous population of Plasmodium falciparum prepared by a single erythrocyte micromanipulation technique was used to produce lines of P. falciparum resistant to mefloquine hydrochloride in vitro. Parasites were maintained in a culture medium containing gradually increased concentrations of mefloquine hydrochloride (CMP-mef) starting with 2 ng/ml. One of the mefloquine-resistant culture lines (W2-mef) was obtained after 96 weeks of continuous culture in CMP-mef, the last 4 weeks in medium containing 40 ng/ml of mefloquine hydrochloride. The W2-mef was four to six times more resistant to mefloquine than was the parent clone W2. Means of multiple determinations of 50% inhibitory concentrations (IC-50) of mefloquine hydrochloride against W2-mef and clone W2 were 20.39 ± 5.08 ng/ml and 4.50 ± 1.94 ng/ml, respectively.