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Improved isolation and crystallization of photosystem I for structural analysis

1998; Elsevier BV; Volume: 1365; Issue: 1-2 Linguagem: Inglês

10.1016/s0005-2728(98)00059-0

1879-2650

Petra Fromme, Horst Tobias Witt,

Mitochondrial Function and Pathology

Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure β-dodecylmaltoside. Crystals of extremely pure PS I are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PS I model derived from X-ray structure analysis at 4 Å resolution (Krauß et al., nat. Struct. Biol. 3 (1996) 965–973; Schubert et al., J. Mol. Biol. 272 (1997) 741–769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Käß, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188–198 and 199–213; Bittl et al., Biochemistry 36 (1997) 12001–12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis.