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Analysis of peptides and proteins affinity-bound to iron oxide nanoparticles by MALDI MS

2007; American Chemical Society; Volume: 18; Issue: 5 Linguagem: Inglês

10.1016/j.jasms.2007.01.011

1879-1123

Sarah Y. Chang, Nan-yan Zheng, Chee‐Shan Chen, Cheng-Dah Chen, Ying‐Yi Chen, C. R. Cris Wang,

Advanced Proteomics Techniques and Applications

Iron oxide nanoparticles modified with oleate have been employed for the extraction of peptides and proteins from aqueous solution before matrix-assisted laser desorption/ionization (MALDI) mass spectrometric (MS) analysis. Adsorption of peptides and proteins onto the nanoparticles were mainly through electrostatic attraction and hydrophobic interaction. The analyte-adsorbed iron oxide nanoparticles could be efficiently collected from solution using a magnet. No elution step was needed. With this preconcentration strategy, the lowest detectable concentration of angiotensin I, insulin, and myoglobin in 500 microL of aqueous solution were 0.1 nM, 0.1 nM, and 10.0 nM, respectively. In addition, the nanoparticles could extract the analytes from solution with a high content of salt and surfactant, thus eliminating suppression effect during MALDI MS analysis. This method was successfully applied to concentrate the tryptic digest products of cytochrome c. In addition, the tryptic digestion of cytochrome c can be directly conducted on the iron oxide nanoparticles.