Toxicity of purothionin and its homologues to the tobacco hornworm, Manduca sexta (L.) (lepidoptera: sphingidae)
1979; Elsevier BV; Volume: 48; Issue: 1 Linguagem: Inglês
10.1016/s0041-008x(79)80020-4
ISSN1096-0333
AutoresKarl J. Kramer, Leland W. Klassen, Berne L. Jones, Roy D. Speirs, Ann E. Kammer,
Tópico(s)Insect Resistance and Genetics
ResumoPurothionin from wheat or homologues from barley and rye were injected into the hemocoel of larvae of Manduca sexta and caused 50% mortality at doses of 17–46 μg/g. Applications at 2–50 μg purothionin to a Manduca moth nerve-flight muscle preparation produced a rapid, dose-dependent depolarization of the muscle-fiber membrane. The α-isopeptides were two- to threefold more toxic than the β-forms. Alkylation of the cysteine residues of the peptides abolished both activities.
Referência(s)