Simultaneous chiral resolution of monosaccharides as 8‐aminonaphthalene‐1,3,6‐trisulfonate derivatives by ligand‐exchange CE using borate as a central ion of the chiral selector
2007; Wiley; Volume: 28; Issue: 21 Linguagem: Inglês
10.1002/elps.200700306
ISSN1522-2683
AutoresShuji Kodama, Sen‐ichi Aizawa, Atsushi Taga, Tomohisa Yamashita, Tomoko Kemmei, Atsushi Yamamoto, Kazuichi Hayakawa,
Tópico(s)Mass Spectrometry Techniques and Applications
ResumoSix reducing monosaccharides (mannose, galactose, fucose, glucose, xylose, and arabinose) were derivatized with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS). Based on the chiral ligand-exchange principle using borate as a central ion of the chiral selector and (S)-3-amino-1,2-propanediol (SAP) as a chiral selector ligand, all of the six ANTS-monosaccharides were simultaneously enantioseparated using absorbance at 245 nm for detection. The optimum conditions for both high resolution and moderately short migration time consisted of 200 mM SAP-200 mM borate buffer (pH 9.2) containing 10% ACN as a BGE at 30 degrees C with an applied voltage of +30 kV. It was revealed that the proposed chiral ligand-exchange CE using the SAP-borate system was applicable to enantioseparation of not only diols but also polyols.
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