Characterization of the EMS1 Gene and its Product, Human Cortactin
1998; Taylor & Francis; Volume: 6; Issue: 2-3 Linguagem: Inglês
10.3109/15419069809004475
ISSN2573-0029
AutoresEd Schuuring, Henk van Damme, Ellen Schuuring-Scholtes, Els Verhoeven, Rob Michalides, Erik Geelen, Carla de Boer, Herbert Brok, Vera Van Buuren, Philip M. Kluin,
Tópico(s)Protease and Inhibitor Mechanisms
ResumoAbstractWe have identified a novel gene, EMSl, that is consistently amplified and overexpressed in human carcinomas with an amplification of the chromosome 11q13 region. Comparisons of the EMSl sequences with those present in the GenBank databases revealed a high identity with chicken cortactin. Southern and western blot analyses confirm the high sequence conservation during evolution. An antiserum specific for human cortactin, showed in gene transfer experiments that both human p80 and p85 isoforms are encoded by the EMSl cDNA. Further comparisons demonstrated an high sequence and structural homology with HSl that is implicated in signal transduction in lymphoid cells only. Expression of EMSl/cortactin mRNA was restricted to tumor cell lines derived from non-lymphoid origin. Cortactin contains (i) a filamentous actin binding tandem repeat domain, (ii) a proline-rich SH3-binding and (iii) a SH3 domain that is common in proteins involved in signal transduction. Our data suggest that human EMSl/cortactin has a function in signal transmission between cell-matrix contact sites and the cytoskeleton and, as such, its overexpression due to 11q13 amplification might effect adhesive properties of human carcinomas.Key Words: Cyclin Dlchromosome 11q13gene amplificationbreast cancer
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