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Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology. Application as HPLC stationary phases

2009; Elsevier BV; Volume: 25; Issue: 7 Linguagem: Inglês

10.1016/j.bios.2009.12.023

1873-4235

Mara Soares da Silva, Eva R. Vão, Márcio Temtem, Luís Mafra, Jorge Caldeira, Ana Aguiar‐Ricardo, Teresa Casimiro,

Microfluidic and Capillary Electrophoresis Applications

Molecularly imprinted polymers (MIPs) of poly(ethylene glycol dimethacrylate) and poly(N-isopropylacrylamide-co-ethylene glycol dimethacrylate) were synthesized for the first time in supercritical carbon dioxide (scCO(2)), using Boc-L-tryptophan as template. Supercritical fluid technology provides a clean and one-step synthetic route for the preparation of affinity polymeric materials with sensing capability for specific molecules. The polymeric materials were tested as stationary HPLC phases for the enantiomeric separation of L- and D-tryptophan. HPLC results prove that the synthesized MIPs are able to recognize the template molecule towards its enantiomer which opens up potential applications in chromatographic chiral separation.