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Measurement of circular dichroism dynamics in a nanosecond temperature-jump experiment

2011; American Institute of Physics; Volume: 82; Issue: 5 Linguagem: Inglês

10.1063/1.3592331

1527-2400

Mai-Thu Khuc, Lucille Mendonça, Sapna Sharma, Xavier Solinas, Martin Völk, F. Hache,

Mass Spectrometry Techniques and Applications

The use of a fast temperature jump (T-jump) is a very powerful experiment aiming at studying protein denaturation dynamics. However, probing the secondary structure is a difficult challenge and rarely yields quantitative values. We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution.