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Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate

1993; Oxford University Press; Volume: 110; Issue: 1 Linguagem: Inglês

10.1111/j.1574-6968.1993.tb06302.x

1574-6968

Martine Malissard, Colette Duez, Micheline Guinand, Marie‐Jeanne Vacheron, Georges Michel, N. Marty, Bernard Joris, Iris Thamm, Jean‐Marie Ghuysen,

Polyamine Metabolism and Applications

The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl.Environ.Microbiol.(1991) 57, 1870-1872).The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase.The 265-amino acid residue alginate lyase showed lyric activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient.Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385-8391).