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Engineering of Thermus thermophilus Cytochrome c 552 : Thermally Tolerant Artificial Peroxidase*

2008; Wiley; Volume: 9; Issue: 18 Linguagem: Inglês

10.1002/cbic.200800599

1439-7633

Hiroshi Nakajima, Yusuke Ichikawa, Yuh Satake, Nobuyuki Takatani, Soumen K. Manna, Jitumani Rajbongshi, Shyamalava Mazumdar, Yoshihito Watanabe,

Enzyme-mediated dye degradation

ChemBioChemVolume 9, Issue 18 p. 2954-2957 Communication Engineering of Thermus thermophilus Cytochrome c552: Thermally Tolerant Artificial Peroxidase* Hiroshi Nakajima Prof. Dr., Hiroshi Nakajima Prof. Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorYusuke Ichikawa Dr., Yusuke Ichikawa Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorYuh Satake Dr., Yuh Satake Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorNobuyuki Takatani Dr., Nobuyuki Takatani Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorSoumen Kanti Manna Dr., Soumen Kanti Manna Dr. Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005 (India)Search for more papers by this authorJitumani Rajbongshi, Jitumani Rajbongshi Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005 (India)Search for more papers by this authorShyamalava Mazumdar Prof. Dr., Shyamalava Mazumdar Prof. Dr. Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005 (India)Search for more papers by this authorYoshihito Watanabe Prof. Dr., Yoshihito Watanabe Prof. Dr. [email protected] Research Center of Materials Science, Nagoya University, Nagoya 464-8602 (Japan), Fax: (+81) 52-789-3049Search for more papers by this author Hiroshi Nakajima Prof. Dr., Hiroshi Nakajima Prof. Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorYusuke Ichikawa Dr., Yusuke Ichikawa Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorYuh Satake Dr., Yuh Satake Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorNobuyuki Takatani Dr., Nobuyuki Takatani Dr. Graduate School of Science, Department of Chemistry, Nagoya University, Nagoya 464-8602 (Japan)Search for more papers by this authorSoumen Kanti Manna Dr., Soumen Kanti Manna Dr. Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005 (India)Search for more papers by this authorJitumani Rajbongshi, Jitumani Rajbongshi Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005 (India)Search for more papers by this authorShyamalava Mazumdar Prof. Dr., Shyamalava Mazumdar Prof. Dr. Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005 (India)Search for more papers by this authorYoshihito Watanabe Prof. Dr., Yoshihito Watanabe Prof. Dr. [email protected] Research Center of Materials Science, Nagoya University, Nagoya 464-8602 (Japan), Fax: (+81) 52-789-3049Search for more papers by this author First published: 09 December 2008 https://doi.org/10.1002/cbic.200800599Citations: 15Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Customizing enzymes: We demonstrate that cytochrome c552 (Cyt c552) from Thermus thermophilus HB8 can be transformed into a thermally tolerant peroxidase by a design process modeled on the catalytic mechanism of peroxidases. At temperatures above 50 °C, the enzymatic activity of the engineered Cyt c552 surpasses that of a myoglobin variant that is known to exhibit the highest activity among artificial peroxidases. Citing Literature Supporting Information Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description cbic_200800599_sm_miscellaneous_information.pdf75.8 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume9, Issue18December 15, 2008Pages 2954-2957 RelatedInformation