Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry
1986; Wiley; Volume: 57; Issue: 1 Linguagem: Inglês
10.1111/j.1768-322x.1986.tb00458.x
ISSN1768-322X
AutoresXavier Ronot, Laurent Benel, M Adolphe, Jean‐Claude Mounolou,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoFlow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of the whole cell and its organelles. Rhodamine 123 is a vital fluorescent dye used in flow cytometry. As it is specifically concentrated in mitochondria because of the transmembrane potential that these organelles maintain in living cells, rhodamine 123 is thus a useful probe for monitoring the abundance and activity of mitochondria. A critical survey of the routine use of rhodamine 123 together with flow cytometry in mitochondrial research is presented.
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