Solubilization of receptor for pituitary adenylate cyclase activating polypeptide from bovine brain
1990; Elsevier BV; Volume: 172; Issue: 2 Linguagem: Inglês
10.1016/0006-291x(90)90732-3
ISSN1090-2104
AutoresYasushi Masuda, Tetsuya Ohtaki, Chieko Kitada, Masao Tsuda, Akira Arimura, Masahiko Fujino,
Tópico(s)Cardiovascular, Neuropeptides, and Oxidative Stress Research
ResumoA receptor for pituitary adenylate cyclase activating polypeptide (PACAP) was solubilized from bovine brain membranes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Saturable binding experiments using [125I]PACAP27 revealed that the solubilized extract contained a single class of binding sites with a Kd of 200 pM and a Bmax of 0.9 pmol/mg protein. The Kd did not change significantly after the solubilization. Competitive binding experiments confirmed that the solubilized receptor retained a specificity for PACAP. Thus, the solubilization of the receptor in an active form was successfully achieved by the present procedure.
Referência(s)