Produção Nacional
Revisado por pares
Assessment of Variation in Bacterial Composition among Microhabitats in a Mangrove Environment Using DGGE Fingerprints and Barcoded Pyrosequencing
2012; Public Library of Science; Volume: 7; Issue: 1 Linguagem: Inglês
10.1371/journal.pone.0029380
ISSN1932-6203
AutoresDaniel F. R. Cleary, Kornelia Smalla, Lêda C. Mendonça-Hagler, Newton C. M. Gomes,
Tópico(s)Protist diversity and phylogeny
ResumoHere, we use DGGE fingerprinting and barcoded pyrosequencing data, at six cut-off levels (85–100%), of all bacteria, Alphaproteobacteria and Betaproteobacteria to assess composition in the rhizosphere of nursery plants and nursery-raised transplants, native plants and bulk sediment in a mangrove habitat. When comparing compositional data based on DGGE fingerprinting and barcoded pyrosequencing at different cut-off levels, all revealed highly significant differences in composition among microhabitats. Procrustes superimposition revealed that ordination results using cut-off levels from 85–100% and DGGE fingerprint data were highly congruent with the standard 97% cut-off level. The various approaches revealed a primary gradient in composition from nursery to mangrove samples. The affinity between the nursery and transplants was greatest when using Betaproteobacteria followed by Alphaproteobacteria data. There was a distinct secondary gradient in composition from transplants to bulk sediment with native plants intermediate, which was most prevalent using all bacteria at intermediate cut-off levels (92–97%). Our results show that PCR-DGGE provides a robust and cost effective exploratory approach and is effective in distinguishing among a priori defined groups.
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