Giardia lamblia : axenic growth in autoclaved and filtered Diamond's TYI-S-33 medium
1982; NRC Research Press; Volume: 60; Issue: 7 Linguagem: Inglês
10.1139/z82-219
ISSN1480-3283
AutoresMiodrag Belosevic, G. M. Faubert, Neil A. Croll, J. D. MacLean,
Tópico(s)Orthopedic Infections and Treatments
ResumoThe trophozoites of Giardia lamblia were gradually adapted to grow in both autoclaved and filtered Diamond's TYI-S-33 culture medium. Comparative growth studies indicated that the growth of organisms was significantly higher in filtered TYI-S-33 medium. In both types of media the exponential growth phase occurred between 48 and 96 h postinoculation. The mean number of trophozoites at 96 h was 1.94 × 10 5 and 4.82 × 10 5 cells/mL for autoclaved and filtered media, respectively. The generation times for the exponential growth phase were 9.66 h. for autoclaved and 7.69 h for filtered medium. The percentage of dead trophozoites was similar in both media and was 14% for the first 8 days postinoculation.
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