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Hydrosoluble formazan XTT: its application to natural products drug discovery for Leishmania

2003; Elsevier BV; Volume: 55; Issue: 3 Linguagem: Inglês

10.1016/j.mimet.2003.08.013

1872-8359

C. Williams, Omar Ariel Espinosa, Héctor Montenegro, Luis Cubilla, Todd L. Capson, Eduardo Ortega-Barrı́a, Luz Romero,

Phytochemistry and biological activities of Ficus species

A colorimetric method for measuring the viability of Leishmania promastigotes is described that is based on the reduction of the tetrazolium salt, XTT, to a water-soluble formazan. Values obtained by the XTT method correlated well with parasite number (r=0.965) and with methods that rely upon the reduction of MTT or MTS (r=0.96 and 0.97, respectively). The IC50 values obtained by XTT method with amphotericin-B, miltefosine and ketoconazole were similar to those previously reported by other methods. The XTT method proved to be a reliable and convenient method for the screening of methanolic extracts from 1059 plants and was used for the bioassay-guided fractionation of the alkaloid aegeline from Sarcorhachis naranjoana.