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ATP for the DNA Ligation Step in Base Excision Repair Is Generated from Poly(ADP-ribose)

2000; Elsevier BV; Volume: 275; Issue: 30 Linguagem: Inglês

10.1074/jbc.m002429200

1083-351X

Shiao Li Oei, Mathias Ziegler,

CRISPR and Genetic Engineering

In mammalian cells, the base excision repair (BER) pathway is the main route to counteract the mutagenic effects of DNA lesions. DNA nicks induce, among others, DNA polymerase activities and the synthesis of poly(ADP-ribose). It is shown here that poly(ADP-ribose) serves as an energy source for the final and rate-limiting step of BER, DNA ligation. This conclusion was drawn from experiments in which the fate of [32P]poly(ADP-ribose) or [32P]NAD added to HeLa nuclear extracts was systematically followed. ATP was synthesized from poly(ADP-ribose) in a pathway that strictly depended on nick-induced DNA synthesis. NAD was used for the synthesis of poly(ADP-ribose), which, in turn, was converted to ATP by pyrophosphorylytic cleavage utilizing the pyrophosphate generated from dNTPs during DNA synthesis. The adenylyl moiety was then preferentially used to adenylate DNA ligase III, from which it was transferred to the 5′-phosphoryl end of the nicked DNA. Finally, ligation to the 3′-OH end resulted in the release of AMP. When using NAD, but not poly(ADP-ribose), in the presence of 3-aminobenzamide, the entire process was blocked, confirming poly(ADP-ribosyl)ation to be the essential initial step. Thus, poly(ADP-ribose) polymerase-1, DNA polymerase β, and ligase III interact with x-ray repair cross-complementing protein-1 within the BER complex, which ensures that ATP is generated and specifically used for DNA ligation. In mammalian cells, the base excision repair (BER) pathway is the main route to counteract the mutagenic effects of DNA lesions. DNA nicks induce, among others, DNA polymerase activities and the synthesis of poly(ADP-ribose). It is shown here that poly(ADP-ribose) serves as an energy source for the final and rate-limiting step of BER, DNA ligation. This conclusion was drawn from experiments in which the fate of [32P]poly(ADP-ribose) or [32P]NAD added to HeLa nuclear extracts was systematically followed. ATP was synthesized from poly(ADP-ribose) in a pathway that strictly depended on nick-induced DNA synthesis. NAD was used for the synthesis of poly(ADP-ribose), which, in turn, was converted to ATP by pyrophosphorylytic cleavage utilizing the pyrophosphate generated from dNTPs during DNA synthesis. The adenylyl moiety was then preferentially used to adenylate DNA ligase III, from which it was transferred to the 5′-phosphoryl end of the nicked DNA. Finally, ligation to the 3′-OH end resulted in the release of AMP. When using NAD, but not poly(ADP-ribose), in the presence of 3-aminobenzamide, the entire process was blocked, confirming poly(ADP-ribosyl)ation to be the essential initial step. Thus, poly(ADP-ribose) polymerase-1, DNA polymerase β, and ligase III interact with x-ray repair cross-complementing protein-1 within the BER complex, which ensures that ATP is generated and specifically used for DNA ligation. base excision repair 3-amionobenzamide DNA ligase methyl-N′-nitro-N′-nitrosoguanidine polyacrylamide gel electrophoresis poly(ADP-ribose) poly(ADP-ribosyl) polymerase-1 DNA polymerase β x-ray repair cross-complementing protein-1 The maintenance of an intact genome is crucial to each individual. Therefore, DNA damages need to be efficiently removed, which is accomplished by complex DNA repair mechanisms (reviewed in Ref. 1Lindahl T. Wood R.D. Science. 1999; 286: 1897-1905Crossref PubMed Scopus (1240) Google Scholar). The major pathway, BER,1 is initiated by DNA glycosylases that cleave the base-deoxyribose glycosyl bond of a damaged nucleotide residue. Then, endonucleases are recruited that cleave the chain on the 5′ side of the abasic site. As a result, nicked DNA intermediates occur. Nicked DNA, in turn, triggers the catalytic activities of DNA polymerase β (Pol β) and poly(ADP-ribose) polymerase-1 (PARP-1) (2Sobol R.W. Horton J.K. Kuhn R. Gu H. Singhal R.K. Prasad R. Rajewsky K. Wilson S.H. Nature. 1996; 379: 183-186Crossref PubMed Scopus (754) Google Scholar, 3Benjamin R.C. Gill D.M. J. Biol. Chem. 1980; 255: 10502-10508Abstract Full Text PDF PubMed Google Scholar, 4Durkacz B.W. Omidiji O. Gray D.A. Shall S. Nature. 1980; 283: 593-596Crossref PubMed Scopus (864) Google Scholar). Several different enzymes with poly(ADP-ribosyl)ation activity (EC2.4.2.30) have been described recently, but the major cellular pathway of NAD catabolism in response to the appearance of DNA lesions has been ascribed to the catalytic activity of the 116-kDa protein PARP-1 (Ref.4Durkacz B.W. Omidiji O. Gray D.A. Shall S. Nature. 1980; 283: 593-596Crossref PubMed Scopus (864) Google Scholar; reviewed in Ref. 5D'Amours D. Desnoyers S. D'Silva I. Poirier G.G. Biochem. J. 1999; 342: 249-268Crossref PubMed Scopus (0) Google Scholar). Besides a potential participation of PARP-1 in transcription (6Oei S.L. Griesenbeck J. Ziegler M. Schweiger M. Biochemistry. 1998; 37: 1465-1469Crossref PubMed Scopus (57) Google Scholar, 7Oei S.L. Griesenbeck J. Schweiger M. Ziegler M. J. Biol. Chem. 1998; 273: 31644-31647Abstract Full Text Full Text PDF PubMed Scopus (108) Google Scholar), recombination, apoptosis, and necrosis (5D'Amours D. Desnoyers S. D'Silva I. Poirier G.G. Biochem. J. 1999; 342: 249-268Crossref PubMed Scopus (0) Google Scholar), a large number of molecular and genetic studies have clearly implicated PARP-1 activity in positively regulating BER (8Molinete M. Vermeulen W. Burkle A. Menissier-de Murcia J. Kupper J.H. Hoeijmakers J.H. de Murcia G. EMBO J. 1993; 12: 2109-2117Crossref PubMed Scopus (221) Google Scholar, 9Dantzer F. Schreiber V. Niedergang C. Trucco C. Flatter E. De La Rubia G. Oliver J. Rolli V. Menissier-de Murcia J. de Murcia G. Biochimie. 1999; 81: 69-75Crossref PubMed Scopus (296) Google Scholar). Originally, it was suggested that poly(ADP-ribosyl)ation may activate a DNA ligase required for DNA repair in mammalian cells (10Creissen D. Shall S. Nature. 1982; 296: 271-272Crossref PubMed Scopus (297) Google Scholar). Several further investigations confirmed a positive influence of PARP-1 activity on DNA repair, especially on DNA ligation (Refs. 11Ding R. Pommier Y. Kang V.H. Smulson M. J. Biol. Chem. 1992; 267: 12804-12812Abstract Full Text PDF PubMed Google Scholar, 12Trucco C. Oliver F.J. de Murcia G. Menissier-de Murcia J. Nucleic Acids Res. 1998; 26: 2644-2649Crossref PubMed Scopus (299) Google Scholar, 13Kubota Y. Nash R.A. Klungland A. Schar P. Barnes D.E. Lindahl T. EMBO J. 1996; 15: 6662-6670Crossref PubMed Scopus (666) Google Scholar; reviewed in Ref. 9Dantzer F. Schreiber V. Niedergang C. Trucco C. Flatter E. De La Rubia G. Oliver J. Rolli V. Menissier-de Murcia J. de Murcia G. Biochimie. 1999; 81: 69-75Crossref PubMed Scopus (296) Google Scholar). However, the actual function of PARP-1 in the BER process and the mechanism whereby poly(ADP-ribose) synthesis stimulates ligation (14Satoh M.S. Lindahl T. Nature. 1992; 356: 356-358Crossref PubMed Scopus (898) Google Scholar) have still remained obscure (reviewed in Ref. 9Dantzer F. Schreiber V. Niedergang C. Trucco C. Flatter E. De La Rubia G. Oliver J. Rolli V. Menissier-de Murcia J. de Murcia G. Biochimie. 1999; 81: 69-75Crossref PubMed Scopus (296) Google Scholar). X-ray repair cross-complementing protein-1 (XRCC1) was the first human gene product isolated that mediates the cellular response to ionizing radiation (15Thompson L.H. Brookman K.W. Jones N.J. Allen S.A. Carrano A.V. Mol. Cell. Biol. 1990; 10: 6160-6171Crossref PubMed Scopus (370) Google Scholar). This protein is apparently essential and required for the BER pathway (16Tebbs R.S. Flannery M.L. Meneses J.J. Hartmann A. Tucker J.D. Thompson L.H. Cleaver J.E. Pedersen R.A. Dev. Biol. 1999; 208: 513-529Crossref PubMed Scopus (286) Google Scholar). Recent investigations demonstrated specific interactions of XRCC1 with Pol β, DNA ligase III (Lig III), and PARP-1 (13Kubota Y. Nash R.A. Klungland A. Schar P. Barnes D.E. Lindahl T. EMBO J. 1996; 15: 6662-6670Crossref PubMed Scopus (666) Google Scholar,17Caldecott K.W. McKeown C.K. Tucker J.D. Ljungquist S. Thompson L.H. Mol. Cell. Biol. 1994; 14: 68-76Crossref PubMed Google Scholar, 18Masson M. Niedergang C. Schreiber V. Muller S. Menissier-de Murcia J. de Murcia G. Mol. Cell. Biol. 1998; 18: 3563-3571Crossref PubMed Scopus (795) Google Scholar, 19Caldecott K.W. Aoufouchi S. Johnson P. Shall S. Nucleic Acids Res. 1996; 24: 4387-4394Crossref PubMed Scopus (526) Google Scholar, 20Cappelli E. Taylor R. Cevasco M. Abbondandolo A. Caldecott K. Frosina G. J. Biol. Chem. 1997; 272: 23970-23975Abstract Full Text Full Text PDF PubMed Scopus (268) Google Scholar). Therefore, the complex of these proteins is supposed to control BER (1Lindahl T. Wood R.D. Science. 1999; 286: 1897-1905Crossref PubMed Scopus (1240) Google Scholar, 9Dantzer F. Schreiber V. Niedergang C. Trucco C. Flatter E. De La Rubia G. Oliver J. Rolli V. Menissier-de Murcia J. de Murcia G. Biochimie. 1999; 81: 69-75Crossref PubMed Scopus (296) Google Scholar, 19Caldecott K.W. Aoufouchi S. Johnson P. Shall S. Nucleic Acids Res. 1996; 24: 4387-4394Crossref PubMed Scopus (526) Google Scholar). Most situations requiring highly efficient DNA repair are accompanied by a dramatic decrease of the cellular ATP concentration. Considering that ligation represents the rate-limiting, ATP-dependent step in BER, it appears reasonable to expect a compensatory mechanism that would enable efficient DNA repair even in situations of energy deprivation. In the present report, it is demonstrated that poly(ADP-ribose) synthesized by PARP-1 may serve as a source of ATP that is specifically used for ligation. This finding provides a molecular mechanism for previous observations, in vivo and in vitro, demonstrating the specific stimulation of ligation by poly(ADP-ribosyl)ation during BER. All reactions were carried out with nuclear HeLa cell extracts purchased from Promega or prepared (21Schreiber E. Matthias P. Müller M.M. Schaffner W. Nucleic Acids Res. 1989; 17: 6419Crossref PubMed Scopus (3880) Google Scholar). In standard reactions (10 mm Hepes, pH 7.9, 10% glycerol, 7 mm MgCl2, 50 mm KCl, 100 mm NaCl, 0, 1 mm EDTA, 0.25 mmdithiothreitol, 0.25 mm phenylmethylsulfonyl fluoride, 10 μg/ml aphidicolin), 8 μg of nuclear protein were incubated with 200 ng of nicked plasmid DNA. Further additions are indicated in the legends to the figures. The reaction volume was 10 μl, and incubations were conducted at 30 °C for the time periods indicated. All data presented are representative of at least three independent experiments. Nicked plasmid was obtained by controlled incubation of an empty vector plasmid, pUC 9, with DNase I and subsequent purification of nicked plasmids by CsCl ethidium bromide centrifugation. Poly(ADP-ribose) was synthesized with 10 μg/ml purified recombinant PARP-1 from 100 μm NAD as described before (22Griesenbeck J. Oei S.L. Mayer-Kuckuk P. Ziegler M. Buchlow G. Schweiger M. Biochemistry. 1997; 36: 7297-7304Crossref PubMed Scopus (43) Google Scholar). Synthesized polymers were freed of residual amounts of DNA by DNase treatment followed by phenol/chloroform extraction and precipitation. Reactions were stopped by precipitation with 10 volumes of acetone. Precipitated nucleotides were redissolved in 10 mm Tris-HCl, pH 8.0, 0.1 mmEDTA. Samples containing equal amounts of radioactivity (as estimated by Cerenkov counting) were subjected to cellulose-coated plates (Machery-Nagel). Chromatography was performed as described before (23Hagen T. Ziegler M. Biochim. Biophys. Acta. 1997; 1340: 7-12Crossref PubMed Scopus (7) Google Scholar) using the solvent system isobutyric acid/25% NH4OH/H2O (96/4/19 (v/v/v)). After separation was completed, the cellulose plates were dried and subjected to autoradiography. Reactions were stopped, and proteins were separated by 6% polyacrylamide gel electrophoresis. For analysis of plasmids, reactions were stopped, and after phenol/chloroform extraction, DNA was precipitated with ethanol, resuspended, and applied to 1% agarose gels in 90 mm Tris, 90 mm boric acid, 2 mm EDTA, pH 8.3, and 50 μg/ml chloroquine. HeLa cells were grown in suspension in RPMI 1640 medium supplemented with 10% fetal calf serum in a humidified 5% CO2 atmosphere at 37 °C. A medium change of confluent cells was performed 2 h before treatment with methyl-N′-nitro-N′-nitrosoguanidine (MNNG). At a density of 106 cells/ml, a 10-ml culture was treated with 100 μm MNNG for 5 min. The cells were then centrifuged and washed twice, and the culture was continued in a culture flask at 37 °C. 1-ml aliquots of the cell culture were taken after the time intervals indicated, and nuclear extracts were prepared (21Schreiber E. Matthias P. Müller M.M. Schaffner W. Nucleic Acids Res. 1989; 17: 6419Crossref PubMed Scopus (3880) Google Scholar). Poly(ADP-ribosyl)ation and nick-induced DNA synthesis activities of nuclear extracts were determined under the same conditions as described above. However, the 10-μl reactions contained only 0.5 μg of nuclear protein. Incorporation of ADP-ribose was measured by using 1 μm [α-32P]NAD (NEN Life Science Products) and incubating for 15 min at ambient temperature. Incubations were stopped by trichloroacetic acid precipitation. Incorporation of32P-labeled ADP-ribose into washed precipitates was determined by Cerenkov counting. Nick-induced DNA synthesis was measured by using 0.1 mm dATP, dGTP, and dTTP and 1 nm [α-32P]dCTP. Incubations were continued for 30 min at 30 °C. The reactions were stopped, and after phenol/chloroform extraction, DNA was precipitated with ethanol, resuspended, and subjected to agarose gel electrophoresis. The gel was dried and subjected to autoradiography. Incorporation of labeled nucleotides was determined by Cerenkov counting of excised gel pieces. All data presented are representative of at least three independent experiments. First, the metabolism of α-32P-labeled deproteinized poly(ADP-ribose) (PAR) incubated in the presence of nicked DNA was analyzed in HeLa nuclear extracts. The major product of PAR degradation is ADP-ribose (Fig. 1 A, lane 1) arising from the activity of poly(ADP-ribose) glycohydrolase activity present in nuclear extracts (24Alvarez-Gonzalez R. Althaus F.R. Mutat. Res. 1989; 218: 67-74Crossref PubMed Scopus (179) Google Scholar, 25Lin W. Ame J.C. Aboul-Ela N. Jacobson E.L. Jacobson M.K. J. Biol. Chem. 1997; 272: 11895-11901Abstract Full Text Full Text PDF PubMed Scopus (181) Google Scholar). In the presence of 1 mm deoxynucleotides (dNTPs), which allowed nick-induced DNA synthesis, in addition to radiolabeled ADP-ribose and AMP, labeled ATP was detected (Fig. 1 A, lane 2). Addition of only one or two unlabeled dNTPs failed to support the generation of labeled ATP. A mixture of at least three dNTPs enabled the synthesis of labeled ATP from labeled PAR (not shown). On the other hand, addition of 1 mm sodium pyrophosphate instead of dNTPs did not give rise to the generation of significant amounts of ATP (Fig. 1 A, lane 3). Thus, synthesis of ATP strictly depended on DNA synthesis. Moreover, the requirement for dNTPs indicated that the pyrophosphate released during DNA synthesis is specifically channeled to the ATP-forming activity, because added pyrophosphate was inefficient. It should be pointed out that the only feasible direct pathway for the generation of ATP from poly(ADP-ribose) includes the cleavage of the phosphodiester bond of ADP-ribose by a pyrophosphorylase, yielding ATP and ribose-5′-phosphate (26Maruta H. Matsumura N. Tanuma S. Biochem. Biophys. Res. Commun. 1997; 236: 265-269Crossref PubMed Scopus (52) Google Scholar). If radioactively labeled ADP-ribose was used in similar reactions, synthesis of ATP was only detected at very high concentrations (10 mm) of pyrophosphate (not shown). The dependence of ATP generation on the presence of ADP-ribose polymers was further supported by incubations of nuclear extracts with dNTPs and α-32P-labeled NAD (Fig. 1 B, lanes 2–4). The synthesis of labeled ATP from labeled NAD was not detected, if the reaction was performed in the presence of the inhibitor of poly(ADP-ribosyl)ation, 3-aminobenzamide (3-AB) (Fig. 1 B, lane 5). However, the presence of 3-AB did not prevent the synthesis of radiolabeled ATP from labeled PAR (Fig. 1 B, lane 10). Consequently, dNTP-dependent formation of ATP exhibited an absolute requirement for PAR, either added directly or synthesized by endogenous PARP activity. ATP generation from labeled NAD or labeled PAR (Fig. 1 B) attained a maximum after about 20 min of incubation (compare in Fig. 1 B, lanes 3 and 8 versus lanes 4 and 9). If an amount of [α-32P]ATP equivalent to that of NAD or ADP-ribose units in PAR was used instead, it was almost entirely metabolized to ADP. Also, in this case, only a very little AMP (<1%) was formed (Fig. 1 C, right lane). In contrast, ATP synthesized from PAR coupled to DNA synthesis was apparently not degraded to ADP (see Fig.1, A and C). This suggests that the PAR-derived ATP is inaccessible to ATPases, such as topoisomerases, helicases, or kinases, but is accessible only to enzymes metabolizing ATP to AMP. Thus, the pathway of ATP synthesis using PAR as intermediate product appeared to provide ATP for a specific reaction. In the first step of DNA ligation, ATP is used to form an adenylated ligase intermediate. In subsequent steps, the adenylyl group is transferred to the 5′-phosphoryl donor, and eventually, AMP is released when the 5′-phosphoryl and the 3′-OH ends are joined. Owing to the fact that both PARP-1 and Lig III are constituents of the BER complex (17Caldecott K.W. McKeown C.K. Tucker J.D. Ljungquist S. Thompson L.H. Mol. Cell. Biol. 1994; 14: 68-76Crossref PubMed Google Scholar, 18Masson M. Niedergang C. Schreiber V. Muller S. Menissier-de Murcia J. de Murcia G. Mol. Cell. Biol. 1998; 18: 3563-3571Crossref PubMed Scopus (795) Google Scholar, 19Caldecott K.W. Aoufouchi S. Johnson P. Shall S. Nucleic Acids Res. 1996; 24: 4387-4394Crossref PubMed Scopus (526) Google Scholar, 20Cappelli E. Taylor R. Cevasco M. Abbondandolo A. Caldecott K. Frosina G. J. Biol. Chem. 1997; 272: 23970-23975Abstract Full Text Full Text PDF PubMed Scopus (268) Google Scholar), it appeared to be a likely possibility that Lig III (molecular weight, 103,000) is specifically using ATP generated from PAR as described above. To test this possibility, nuclear extracts were incubated in the presence of nicked DNA, dNTPs, and α-32P-labeled NAD for several time intervals, similarly to reactions shown in Fig. 1 B, lanes 2–4. Proteins were then separated by SDS-PAGE and subsequently subjected to autoradiography (Fig. 1 D). As expected, the major radioactively labeled protein represented automodified endogenous PARP-1 (molecular weight, 116,000) or PARP-1-fragments (Fig. 1 D, asterisks), in accordance with previous reports showing that the predominant reaction of poly(ADP-ribosyl)ation represents automodification of PARP-1 itself (27Ogata N. Ueda K. Kawaichi M. Hayaishi O. J. Biol. Chem. 1981; 256: 4135-4137Abstract Full Text PDF PubMed Google Scholar). A further radiolabeled protein with an apparent molecular weight of about 100,000 was detected (Fig.1 D, arrow on right), the modification of which was sensitive to treatment with acid (Fig. 1 D, lane 4, arrow on right). Compared with poly(ADP-ribosylation), adenylation of proteins is relatively unstable, especially at acidic pH. Incubation of nuclear extracts with [α-32P]ATP and separation of proteins on SDS-PAGE followed by autoradiography visualized proteins modified by adenylation (Fig. 1 E, left lane). As expected, several adenylated proteins exhibited apparent molecular masses corresponding to those of known human DNA ligases (28Tomkinson A.E. Levin D.L. BioEssays. 1997; 19: 893-901Crossref PubMed Scopus (97) Google Scholar), i.e. Lig I (125 kDa), Lig III (103 kDa), and Lig II (70 kDa) (Fig. 1 E, left lane). If, prior to the adenylation reaction using [α-32P]ATP, unlabeled NAD and dNTPs were incubated with the nuclear extract, the pattern of modified proteins was similar, except that the 103-kDa protein (most likely Lig III) was not labeled. Obviously, this protein had already been adenylated by unlabeled ATP formed during the preincubation in the presence of NAD and dNTPs. Modification of Lig III directly from dNTPs or NAD was excluded by adding an inhibitor of poly(ADP-ribosyl)ation: if the preincubation with NAD and dNTPs was conducted in the presence of 3-AB, labeling of the 103-kDa protein with [α-32P]ATP was undiminished (not shown). The subsequent step after adenylation of DNA ligases is the activation of the donor DNA by transferring the adenylyl group to the phosphorylated 5′-end of a DNA nick. The resulting DNA-AMP complex then reacts with the 3′-OH acceptor group, leading to the ligation of the phosphorylated 5′-end with the 3′-OH under release of AMP. The intermediate DNA-AMP complex can be trapped, if the final joining step of the ligation is prevented. This was accomplished by using 2′-3′-dideoxy NTPs. The specific synthesis of ATP from PAR (see Fig. 1) was still significant, if a mixture of ddATP, ddCTP, dGTP, and dTTP was used instead of dNTPs (not shown). Therefore, during nick-induced DNA synthesis, the incorporation of ddAMP or ddCMP into nicked plasmids would lead, in part, to DNA-AMP intermediates of nicked plasmids with 2′-3′-dideoxy-ends adjacent to an adenylated 5′-end. This possibility was verified in ligation reactions in nuclear extracts using nicked DNA, α-32P-labeled NAD, and unlabeled ddATP, ddCTP, dGTP, and dTTP (Fig. 1 F). The formation of labeled DNA-[32P]AMP complexes was clearly detectable, but only in the absence of 3-AB (Fig. 1 F), that is, only if poly([α-32P]ADP-ribosyl)ation was allowed to occur. These observations provide direct evidence for the conclusion that the adenylyl moieties of poly(ADP-ribose) may be used to activate the 5′-phosphoryl ends of nicked DNA. In this NAD-dependent pathway, the activities of poly(ADP-ribosyl)ation, DNA synthesis, and ligation contribute directly to the ligation of nicked DNA only when combined together. All the enzymes implicated in this mechanism (PARP-1, Pol β, and Lig III) interact with the scaffold protein XRCC1 within the BER complex. Heterodimerization of XRCC1 and Lig III leads to enhanced ligation activity (20Cappelli E. Taylor R. Cevasco M. Abbondandolo A. Caldecott K. Frosina G. J. Biol. Chem. 1997; 272: 23970-23975Abstract Full Text Full Text PDF PubMed Scopus (268) Google Scholar). On the other hand, interaction of XRCC1 with either PARP-1 or Pol β results in a down-regulation of the respective catalytic activity (13Kubota Y. Nash R.A. Klungland A. Schar P. Barnes D.E. Lindahl T. EMBO J. 1996; 15: 6662-6670Crossref PubMed Scopus (666) Google Scholar, 18Masson M. Niedergang C. Schreiber V. Muller S. Menissier-de Murcia J. de Murcia G. Mol. Cell. Biol. 1998; 18: 3563-3571Crossref PubMed Scopus (795) Google Scholar). It was confirmed in this study that in cultured HeLa cells during BER Pol β and PARP-1 activities are indeed similarly regulated (Fig. 2 A). Treatment of HeLa cells with 100 μm MNNG, a well known inductor of the BER pathway, led to transient activation of both PARP-1 and Pol β activities followed by a sharp decline after about 30 min. The assay of DNA synthesis used in this study was restricted to Pol β activity, because other known DNA polymerases were inhibited by aphidicolin (29Friedberg E.C. DNA Repair. W. H. Freeman & Co., New York1985Google Scholar). As tested for PARP-1 in quantitative Western blot analyses (not shown), the modulation of activity was not caused by changes of protein expression. This holds true also for the reactivation after about 3 h following the MNNG treatment. Thesein vivo experiments support the conclusion of previous studies that during DNA repair both PARP-1 and Pol β activities are regulated in concert by their interaction with XRCC1. The time course of the activation of PARP-1 and Pol β also paralleled the enhanced occurrence of poly(ADP-ribose) and depletion of the cellular NAD, ATP, and dNTP pools reported in earlier studies (30Das S.K. Berger N.A. Biochem. Biophys. Res. Commun. 1986; 137: 1153-1158Crossref PubMed Scopus (26) Google Scholar, 31Sims J.L. Berger S.J. Berger N.A. Biochemistry. 1983; 22: 5188-5194Crossref PubMed Scopus (162) Google Scholar). The onset of the final step of BER, ligation, coincides with these events. The ligation step is most important for successful DNA repair; it is rate-limiting and ATP-dependent. The data presented so far suggested that synthesis of poly(ADP-ribose) may serve as an emergency device to complete DNA repair under conditions of cellular ATP depletion. It would be expected then that availability of sufficient ATP should suppress such a pathway. This hypothesis was tested by adding 5 mm ATP to incubations of HeLa nuclear extracts in the presence of nicked DNA, dNTPs, and α-32P-labeled NAD. Surprisingly, under these conditions, modification of nuclear proteins other than PARP-1 or its fragments was not just inhibited but virtually absent (Fig. 2 B, left panel, lane 2), as opposed to the poly(ADP-ribosyl)ation observed in the absence of ATP (lane 1, cf. also Fig. 1 D). Moreover, 32P adenylation of Lig III was also not detectable. Analysis of radiolabeled nucleotides confirmed that in the presence of 5 mm ATP, hardly any of the added [α-32P]NAD was metabolized (Fig. 2 B, right panel, lane 2). Addition of other NTPs, dNTPs, ADP, or AMP at the same concentration (5 mm) did not cause any comparable effect. A direct effect of ATP on PARP-1 was also excluded, because the catalytic activity of the isolated enzyme did not exhibit such a sensitivity toward ATP (not shown). Moreover, addition of isolated recombinant automodification domain of PARP-1 (amino acids 337–573) resulted in the partial recovery of poly(ADP-ribosyl)ation in the presence of ATP (Fig. 2 B, lanes 3). However, adenylation of Lig III was still not detectable. The automodification domain of PARP-1 is known to mediate specific interaction with partner proteins (22Griesenbeck J. Oei S.L. Mayer-Kuckuk P. Ziegler M. Buchlow G. Schweiger M. Biochemistry. 1997; 36: 7297-7304Crossref PubMed Scopus (43) Google Scholar), in particular XRCC1 (18Masson M. Niedergang C. Schreiber V. Muller S. Menissier-de Murcia J. de Murcia G. Mol. Cell. Biol. 1998; 18: 3563-3571Crossref PubMed Scopus (795) Google Scholar). Therefore, it appears that excess of this domain affected the binding of the endogenous PARP-1 to XRCC1, and thus to the BER complex. Consequently, PARP-1 inhibition was abolished, but the BER complex was disabled to synthesize ATP. The suggested effect of the added automodification domain of PARP-1 was further supported by analysis of nick-induced DNA synthesis and ligation catalyzed by the nuclear extracts. Whereas DNA synthesis, but no ligation, was observed in the absence of ATP (Fig. 2 C, lanes 1), the presence of 5 mm ATP led to ligation but suppressed nick-induced DNA synthesis (Fig. 2 C, lanes 2). Addition of the automodification domain of PARP-1 restored nick-induced DNA synthesis in the presence of ATP. Still, the ligated plasmid (closed circle) did not contain radiolabel of the added dNTPs (Fig.2 C, lanes 3). It is well known that DNA repair processes need a high level of ATP. Therefore, in vitro assays are usually conducted in the presence of about 2 mm ATP and a regenerating system (32Wood R.D. Robins P. Lindahl T. Cell. 1988; 53: 97-106Abstract Full Text PDF PubMed Scopus (378) Google Scholar). It was demonstrated in the experiment shown in Fig. 2 D that in the absence of any added ATP or an ATP regenerating system nicked plasmids can be rejoined by HeLa nuclear extracts. However, rejoining occurs only if the conditions established above for the ATP synthesis from PAR are met. That is, conversion of nicked circles to closed circles was only observed if PAR (either directly added or formed from NAD by endogenous PARP-1) and dNTPs were present (Fig. 2 D, lanes 5 and 6). As mentioned before, added ADP-ribose did not serve as a substitute for PAR. The results of the present study support the conclusion that poly(ADP-ribosyl)ation may directly contribute to the process of BER by providing a source of ATP for the ligation step. It is important to note that the favorable effects of NAD and PARP on ligation have long been known. Originally, the observed stimulation of strand break rejoining was suspected to be caused by poly(ADP-ribosyl)ation of a ligase (10Creissen D. Shall S. Nature. 1982; 296: 271-272Crossref PubMed Scopus (297) Google Scholar). However, this suggestion has not been confirmed. In initial in vivo studies, antisense RNA expression was used to deplete cells of PARP-1. It was observed that the absence of PARP-1 resulted in a significant delay of DNA strand break rejoining (11Ding R. Pommier Y. Kang V.H. Smulson M. J. Biol. Chem. 1992; 267: 12804-12812Abstract Full Text PDF PubMed Google Scholar). Moreover, analyses of cells derived from PARP-1–/– mice also revealed a substantial reduction of the DNA ligation activity (12Trucco C. Oliver F.J. de Murcia G. Menissier-de Murcia J. Nucleic Acids Res. 1998; 26: 2644-2649Crossref PubMed Scopus (299) Google Scholar). In the meantime, the BER complex has been well studied, and direct interactions of the proteins involved have been established, both in vitro and in vivo. It was clearly demonstrated that NAD, which provides the substrate for poly(ADP-ribosyl)ation, accelerates the ligation step without influencing, for example, Pol β activity (13Kubota Y. Nash R.A. Klungland A. Schar P. Barnes D.E. Lindahl T. EMBO J. 1996; 15: 6662-6670Crossref PubMed Scopus (666) Google Scholar). Considering the dramatic decrease of the cellular ATP concentration following exposure to genotoxic agents (30Das S.K. Berger N.A. Biochem. Biophys. Res. Commun. 1986; 137: 1153-1158Crossref PubMed Scopus (26) Google Scholar, 31Sims J.L. Berger S.J. Berger N.A. Biochemistry. 1983; 22: 5188-5194Crossref PubMed Scopus (162) Google Scholar), a role of poly(ADP-ribosyl)ation may be the extraction of energy from NAD and its immediate use for DNA repair, specifically ligation. Along this line, the high affinity of PARP-1 for DNA nicks would ensure that NAD is preferentially used at sites in need of DNA repair. Taking into account previous reports proposing the existence of a functional complex consisting of XRCC1, DNA polymerase β, Lig III, and PARP-1 (see Ref.9Dantzer F. Schreiber V. Niedergang C. Trucco C. Flatter E. De La Rubia G. Oliver J. Rolli V. Menissier-de Murcia J. de Murcia G. Biochimie. 1999; 81: 69-75Crossref PubMed Scopus (296) Google Scholar for review), the capability of these constituents of the BER complex to produce ATP from poly(ADP-ribose) provides a rationale for the tight interaction of these proteins (Fig. 3). In addition, the stimulatory influence of NAD specifically on the ligation reaction can be attributed to the generation of ATP, which is apparently directly channeled to the ligase. According to the data presented, the pathway of ATP synthesis does not necessarily include the cleavage of poly(ADP-ribose) to ADP-ribose. However, ADP-ribose may be generated within the tight complex of participating enzymes and may not be substituted for by added ADP-ribose, similar to the situation found for pyrophosphate (Fig.1 A, lane 3). ATP formation using pyrophosphate generated from dNTPs during nick-induced DNA synthesis is difficult to demonstrate directly, because the γ-phosphate group of only a single added [γ-32P]dNTP is readily detectable in ATP (not shown), presumably owing to transphosphorylation of ADP in the nuclear extracts. Nevertheless, the requirement of DNA synthesis to generate ATP from PAR strongly suggests the involvement of pyrophosphate. Furthermore, high concentrations (10 mm) of added pyrophosphate were effective to synthesize ATP from PAR in the absence of dNTPs (not shown). Therefore, it would appear that the detected ATP synthesis includes pyrophosphorolytic cleavage of ADP-ribose, yielding ribose phosphate as its second product. An enzymatic activity catalyzing this kind of reaction has been detected previously in HeLa cell extracts (26Maruta H. Matsumura N. Tanuma S. Biochem. Biophys. Res. Commun. 1997; 236: 265-269Crossref PubMed Scopus (52) Google Scholar). It is of importance to the emerging model (Fig. 3) that the specific generation of ATP requires the synthesis of DNA, which is accompanied by the liberation of pyrophosphate. It is this ATP that is at least preferentially used by Lig III for its autoadenylation and subsequent activation of the DNA donor (5′-phosphorylated end) and, thus, mediates ligation. Moreover, the final phase of BER, including nick-induced DNA synthesis, generation of ATP from PAR, and ligation, appears to be accomplished by an autonomous complex. Bulk phase ATP and pyrophosphate are apparently not used by this complex. Rather, a high cellular energy state (that is, high ATP concentrations) may possibly serve as a signal that favors direct ligation of nicked DNA or execution of the Pol β-dependent short-patch repair pathway (2Sobol R.W. Horton J.K. Kuhn R. Gu H. Singhal R.K. Prasad R. Rajewsky K. Wilson S.H. Nature. 1996; 379: 183-186Crossref PubMed Scopus (754) Google Scholar, 33Dianov G.L. Prasad R. Wilson S.H. Bohr V.A. J. Biol. Chem. 1999; 274: 13741-13743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar). An important observation (Fig. 2 B) relates to the potential function of Lig III and PARP-1 as molecular nick sensors. Both proteins contain highly similar zinc-finger motifs that exhibit high affinity to single strand breaks (34Mackey Z.B. Niedergang C. Menissier-de Murcia J., M. Leppard J. Au K. Chen J. de Murcia G. Tomkinson A.E. J. Biol. Chem. 1999; 274: 21679-21687Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar). According to the data presented in Fig. 2,B and C, at high ATP concentrations, Lig III is readily adenylated. At the same time, poly(ADP-ribosyl)ation and DNA synthesis are strongly inhibited. A possible explanation would be that adenylation of Lig III within the BER complex enhances its affinity to DNA nicks or at least causes the BER complex to assume a conformation that prevents DNA binding of PARP-1 and thereby poly(ADP-ribosyl)ation. Such a mechanism would restrict the use of NAD as an energy source to emergency situations of ATP shortage. We thank G. Buchlow for technical assistance.