EVALUATION OF GENE TRANSFER EFFICIENCY BY VIRAL VECTORS TO MURINE BLADDER EPITHELIUM
2001; Lippincott Williams & Wilkins; Volume: 165; Issue: 2 Linguagem: Inglês
10.1097/00005392-200102000-00091
ISSN1527-3792
AutoresD. Robert Siemens, J. Christopher Austin, William A. See, James Tartaglia, Timothy L. Ratliff,
Tópico(s)Poxvirus research and outbreaks
ResumoNo AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Feb 2001EVALUATION OF GENE TRANSFER EFFICIENCY BY VIRAL VECTORS TO MURINE BLADDER EPITHELIUM D. ROBERT SIEMENS, J. CHRISTOPHER AUSTIN, WILLIAM A. SEE, JAMES TARTAGLIA, and TIMOTHY L. RATLIFF D. ROBERT SIEMENSD. ROBERT SIEMENS More articles by this author , J. CHRISTOPHER AUSTINJ. CHRISTOPHER AUSTIN More articles by this author , WILLIAM A. SEEWILLIAM A. SEE More articles by this author , JAMES TARTAGLIAJAMES TARTAGLIA More articles by this author , and TIMOTHY L. RATLIFFTIMOTHY L. RATLIFF More articles by this author View All Author Informationhttps://doi.org/10.1097/00005392-200102000-00091AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: In pre-clinical gene therapy studies of bladder cancer there is tremendous variation in the ability of viral vectors to deliver genetic material to bladder epithelium. Possible explanations for this variability may involve the physical parameters of delivering vectors in these experimental models. We examined the effects of intravesical volume and pressure during instillation as well as chemical modification of the bladder epithelium on subsequent gene expression in the bladder in mice. Materials and Methods: Female C57B1/6 mice underwent intravesical instillation of the replication restricted canarypox virus (ALVAC) recombinant for the reporter genes luciferase or β-galactosidase. Similar viral titers were instilled at different volumes and a pressure transducer measured intravesical pressure when the vector was instilled. Also, various agents, including 0.6 N hydrochloric acid, 0.4% oxychlorosene, poly-L-lysine and 0.25 M. ammonium chloride, were used to modify the bladder surface before vector instillation and then assayed for transgene expression. Results: As expected, maximum intravesical pressure measured during instillation was significantly greater in mice instilled with a higher volume (33.1 versus 9.8 mm. Hg). Significantly more gene expression was detected in bladders instilled with a higher volume of viral vectors (p <0.05). Likewise, higher instillation pressures resulted in higher transgene expression in distant organs. Modification of the bladder epithelium with agents such as oxychlorosene and poly-L-lysine resulted in elevated gene expression with only minimal increases in systemic activity. Conclusions: Significant differences in gene expression are achieved by varying physical parameters during intravesical instillation. Increased gene expression associated with larger volume instillation may be responsible for some reported variability of gene transfer to the bladder. 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Google Scholar From the Department of Urology and Cancer Center, University of Iowa and Prostate Cancer Research Group, Iowa City, Iowa, and Virogenetics Corp., Troy, New York© 2001 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byChan E, Patel A, Smith A, Klein J, Thomas A, Heston W and Larchian W (2018) Optimizing Orthotopic Bladder Tumor Implantation in a Syngeneic Mouse ModelJournal of Urology, VOL. 182, NO. 6, (2926-2931), Online publication date: 1-Dec-2009.Siemens D (2018) Viral Mediated Gene Therapy for Prostate Cancer—Can We Deliver?Journal of Urology, VOL. 175, NO. 5, (1600-1601), Online publication date: 1-May-2006.SIEMENS D, CRIST S, AUSTIN J, TARTAGLIA J and RATLIFF T (2018) Comparison of Viral Vectors: Gene Transfer Efficiency and Tissue Specificity in a Bladder Cancer ModelJournal of Urology, VOL. 170, NO. 3, (979-984), Online publication date: 1-Sep-2003.van der Poel H, Molenaar B, van Beusechem V, Haisma H, Rodriguez R, Curiel D and Gerritsen W (2018) Epidermal Growth Factor Receptor Targeting of Replication Competent Adenovirus Enhances Cytotoxicity in Bladder CancerJournal of Urology, VOL. 168, NO. 1, (266-272), Online publication date: 1-Jul-2002. Volume 165Issue 2February 2001Page: 667-671 Advertisement Copyright & Permissions© 2001 by American Urological Association, Inc.Keywordsbladder neoplasmsgene therapybladderglycosaminoglycansmiceMetricsAuthor Information D. ROBERT SIEMENS More articles by this author J. CHRISTOPHER AUSTIN More articles by this author WILLIAM A. SEE More articles by this author JAMES TARTAGLIA More articles by this author TIMOTHY L. RATLIFF More articles by this author Expand All Advertisement PDF downloadLoading ...
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