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BIBTEX RIS

Determination of Flavonoids and Resveratrol in Wine by Turbulent-Flow Chromatography-LC-MS

2009; Springer Science+Business Media; Volume: 69; Issue: S2 Linguagem: Inglês

10.1365/s10337-009-1132-x

ISSN

1612-1112

Autores

Michele Antoniuk Presta, Ben Bruyneel, Renato Zanella, Jeroen Kool, Johannes G. Krabbe, H. Lingeman,

Tópico(s)

Sirtuins and Resveratrol in Medicine

Resumo

Turbulent-flow chromatography (TFC) on-line coupled to liquid chromatography mass spectrometry (LC-MS) is used to determine flavonoids and resveratrol in different types of wines. A fully automated system was developed in which 10 mL of sample (diluted wine) was passed over a TFC column, after which the retained analytes were separated by reversed-phase LC and detected by negative ion mode atmospheric-pressure chemical ionization (APCI) MS. The method proved to be fast, non-laborious, robust and sensitive. The feasibility of the method was tested on several red, white and rose wines. Quantitation of resveratrol was possible using the standard addition procedure. Red wine showed the highest amount of resveratrol (4 mg L(-1)), while rose and white wine contained concentrations which were about ten fold lower.

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