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Micellar Electrokinetic Chromatography of Cephalosporin Antibiotics

1996; Wiley; Volume: 43; Issue: 5 Linguagem: Inglês

10.1002/jccs.199600057

2192-6549

Su‐Hwei Chen, Hsin‐Lung Wu, Shou‐Mei Wu, Hwang‐Shang Kou, Shun‐Jin Lin,

Analytical Chemistry and Chromatography

Abstract A simple micellar electrokinetic chromatographic (MEKC) method is described for the simultaneous separation of twelve cephalosporin antibiotics. The separation was performed in a phosphate buffer (0.1 M, pH 6.6) with sodium dodecyl sulfate (SDS) (0.1 M) as an anionic surfactant. The complete separation of twelve cephalosporins was attained in 36 min. Application of the method to the quantitative analysis of cefuroxime, cephalexin, cephapirin and cephradine in pharmaceutical preparations was demonstrated.