Portal de Periódicos da CAPES

Emergence of <i>Salmonella enterica</i> Serovar Infantis Harboring IncI1 Plasmid with <i>bla</i>_CTX-M-14 in a Broiler Farm in Japan

2012; Japanese Society of Veterinary Science; Volume: 74; Issue: 9 Linguagem: Inglês

10.1292/jvms.11-0488

1347-7439

Mitsuhiro Kameyama, Takehisa Chuma, Tomoki YOKOI, Junko Yabata, Kiyoshi Tominaga, Daisuke Miyasako, Hiroyuki Iwata, Karoku OKAMOTO,

Vibrio bacteria research studies

Cefotaxime (CTX)-resistant and -susceptible Salmonella enterica serovar Infantis isolates obtained from broilers raised on a farm in January 2010 in Japan were characterized to establish their resistance determinants. The CTX-resistant isolates produced CTX-M-14 extended-spectrum β-lactamase and harbored 2 distinct plasmid of approximately 140- and 95-kb, whereas the CTX-susceptible isolates harbored one 140-kb plasmid. The 95-kb plasmids were replicon typed as IncI1 carrying the blaCTX-M-14 gene, while the 140-kb plasmids were IncP and harbored the aphA1, aadA1, tetA, and sul1 genes. Genetic fingerprinting by pulsed-field gel electrophoresis revealed similar macrorestriction profiles amongst CTX-resistant and susceptible isolates, suggesting a clonal relationship. The presence of CTX-resistant S. Infantis on a broiler farm has occurred through the acquisition of IncI1 resistance plasmid.