Electrochemical DNA biosensor for analysis of wastewater samples
2002; Elsevier BV; Volume: 58; Issue: 1 Linguagem: Inglês
10.1016/s1567-5394(02)00133-0
ISSN1878-562X
AutoresFausto Lucarelli, Alessandra Kicela, Ilaria Palchetti, Giovanna Marrazza, Marco Mascini,
Tópico(s)bioluminescence and chemiluminescence research
ResumoThe application of a disposable electrochemical DNA biosensor to wastewater samples is reported. The DNA biosensor is assembled by immobilising double-stranded calf thymus DNA on the surface of a disposable, carbon screen-printed electrode (SPE). The oxidation signal of the guanine base, obtained by a square wave voltammetric scan, is used as analytical signal. The presence of compounds with affinity for DNA is measured by their effect on the guanine oxidation. The comparison of the results with a toxicity test based on bioluminescent bacteria has confirmed the applicability of the method to real samples.
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