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Comparative evaluation of the ARCHITECT Toxo IgG, IgM, and IgG Avidity assays for anti-Toxoplasma antibodies detection in pregnant women sera

2009; Elsevier BV; Volume: 65; Issue: 3 Linguagem: Inglês

10.1016/j.diagmicrobio.2009.07.013

1879-0070

Françoise Gay‐Andrieu, Hélène Fricker‐Hidalgo, Eva Sickinger, Anne Espern, Marie‐Pierre Brenier‐Pinchart, Hans-Bertram Braun, Hervé Pelloux,

Cytomegalovirus and herpesvirus research

We assessed the performance of the ARCHITECT Toxo IgG, IgM, and IgG Avidity assays against corresponding assays on AxSYM and Vidas using 730 sera from pregnant women. The ARCHITECT Toxo IgG and IgM assays showed a relative sensitivity of 97.5% and 89.9% and a relative specificity of 99.1% and 99.8%, respectively. If IgM sensitivity is calculated only for sera drawn less than 4 months after infection, the relative sensitivity rises to 98.1%. Correlation between the ARCHITECT and Vidas Avidity assays was 0.87 (n = 103). Testing 86 IgG-positive specimens from recent infection ( 4 months) exhibited high avidity results in 72.5% (137/189) of cases. The method can be used reliably to exclude recent infections in sera with concurrently positive results for IgM and IgG (IgG, >3 IU/mL).