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Catalytic Mechanism and Role of Hydroxyl Residues in the Active Site of Theta Class Glutathione S-Transferases

1997; Elsevier BV; Volume: 272; Issue: 47 Linguagem: Inglês

10.1074/jbc.272.47.29681

1083-351X

Anna Maria Caccuri, Giovanni Antonini, Maria Rita Nicotra, Andrea Battistoni, Mario Lo Bello, Philip G. Board, Michael W. Parker, Giorgio Ricci,

Genomics, phytochemicals, and oxidative stress

Spectroscopic and kinetic studies have been performed on the Australian sheep blowfly Lucilia cuprina glutathione S-transferase (Lucilia GST; EC 2.5.1.18) to clarify its catalytic mechanism. Steady state kinetics of Lucilia GST are non-Michaelian, but the quite hyperbolic isothermic binding of GSH suggests that a steady state random sequential Bi Bi mechanism is consistent with the anomalous kinetics observed. The rate-limiting step of the reaction is a viscosity-dependent physical event, and stopped-flow experiments indicate that product release is rate-limiting. Spectroscopic and kinetic data demonstrate thatLucilia GST is able to lower the pK a of the bound GSH from 9.0 to about 6.5. Based on crystallographic suggestions, the role of two hydroxyl residues, Ser-9 and Tyr-113, has been investigated. Removal of the hydroxyl group of Ser-9 by site-directed mutagenesis raises the pK a of bound GSH to about 7.6, and a very low turnover number (about 0.5% of that of wild type) is observed. This inactivation may be explained by a strong contribution of the Ser-9 hydroxyl group to the productive binding of GSH and by an involvement in the stabilization of the ionized GSH. This serine residue is highly conserved in the Theta class GSTs, so the present findings may be applicable to all of the family members.Tyr-113 appears not to be essential for the GSH activation. Stopped-flow data indicate that removal of the hydroxyl group of Tyr-113 does not change the rate-limiting step of reaction but causes an increase of the rate constants of both the formation and release of the GSH conjugate. Tyr-113 resides on α-helix 4, and its hydroxyl group hydrogen bonds directly to the hydroxyl of Tyr-105. This would reduce the flexibility of a protein region that contributes to the electrophilic substrate binding site; segmental motion of α-helix 4 possibly modulates different aspects of the catalytic mechanism of theLucilia GST. Spectroscopic and kinetic studies have been performed on the Australian sheep blowfly Lucilia cuprina glutathione S-transferase (Lucilia GST; EC 2.5.1.18) to clarify its catalytic mechanism. Steady state kinetics of Lucilia GST are non-Michaelian, but the quite hyperbolic isothermic binding of GSH suggests that a steady state random sequential Bi Bi mechanism is consistent with the anomalous kinetics observed. The rate-limiting step of the reaction is a viscosity-dependent physical event, and stopped-flow experiments indicate that product release is rate-limiting. Spectroscopic and kinetic data demonstrate thatLucilia GST is able to lower the pK a of the bound GSH from 9.0 to about 6.5. Based on crystallographic suggestions, the role of two hydroxyl residues, Ser-9 and Tyr-113, has been investigated. Removal of the hydroxyl group of Ser-9 by site-directed mutagenesis raises the pK a of bound GSH to about 7.6, and a very low turnover number (about 0.5% of that of wild type) is observed. This inactivation may be explained by a strong contribution of the Ser-9 hydroxyl group to the productive binding of GSH and by an involvement in the stabilization of the ionized GSH. This serine residue is highly conserved in the Theta class GSTs, so the present findings may be applicable to all of the family members. Tyr-113 appears not to be essential for the GSH activation. Stopped-flow data indicate that removal of the hydroxyl group of Tyr-113 does not change the rate-limiting step of reaction but causes an increase of the rate constants of both the formation and release of the GSH conjugate. Tyr-113 resides on α-helix 4, and its hydroxyl group hydrogen bonds directly to the hydroxyl of Tyr-105. This would reduce the flexibility of a protein region that contributes to the electrophilic substrate binding site; segmental motion of α-helix 4 possibly modulates different aspects of the catalytic mechanism of theLucilia GST. Glutathione S-transferases (GSTs; 1The abbreviations used are: GST, glutathioneS-transferase; CDNB, 1-chloro-2,4-dinitrobenzene; FDNB, 1-fluoro-2,4-dinitrobenzene. EC 2.5.1.18) are a wide group of isoenzymes able to catalyze the conjugation of GSH with a variety of electrophilic molecules (1Mannervik B. Adv. Enzymol. Relat. Areas Mol. Biol. 1985; 57: 357-417PubMed Google Scholar, 2Mannervik B. Danielson U.H. CRC Crit. Rev. Biochem. 1988; 23: 283-337Crossref PubMed Scopus (1693) Google Scholar, 3Coles B. Ketterer B. CRC Crit. Rev. Biochem. 1990; 25: 47-70Crossref Scopus (435) Google Scholar, 4Armstrong R.N. Chem. Res. Toxicol. 1991; 4: 131-140Crossref PubMed Scopus (326) Google Scholar, 5Armstrong R.N. Adv. Enzymol. Relat. Areas Mol. Biol. 1994; 69: 1-44PubMed Google Scholar, 6Wilce M.C.J. Parker M.W. Biochim. Biophys. Acta. 1994; 1205: 1-18Crossref PubMed Scopus (551) Google Scholar). The cytosolic GSTs are dimeric enzymes subdivided into at least five main classes, Alpha, Mu, Pi (7Mannervik B. Alin P. Guthenberg C. Jensson H. Tahir M.K. Warholm M. Jornvall H. Proc. Natl. Acad. Sci. U. S. A. 1985; 82: 7202-7206Crossref PubMed Scopus (1078) Google Scholar), Theta (8Meyer D.J. Coles B. Pemble S.E. Gilmore K.S. Fraser G.M. Ketterer B. Biochem. J. 1991; 274: 409-414Crossref PubMed Scopus (750) Google Scholar), and Sigma (9Meyer D.J. Thomas M. Biochem. J. 1995; 311: 739-742Crossref PubMed Scopus (144) Google Scholar, 10Ji X. von Rosenvinge E.C. Johnson W.W. Tomarev S.I. Piatigorsky J. Armstrong R.N. Gilliland G.L. Biochemistry. 1995; 34: 5317-5328Crossref PubMed Scopus (217) Google Scholar), and are characterized by a low sequence homology (less than 30%). Despite this heterogeneity, the overall polypeptide fold is very similar among the crystal structures so far obtained (6Wilce M.C.J. Parker M.W. Biochim. Biophys. Acta. 1994; 1205: 1-18Crossref PubMed Scopus (551) Google Scholar), and all GSTs are highly selective for the GSH molecule. An important conserved residue between classes is a tyrosine near the N-terminal region (Tyr-6 in rat GST M1–1 (11Liu S. Zhang P. Ji X. Johnson W.W. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1992; 267: 4296-4299Abstract Full Text PDF PubMed Google Scholar), Tyr-7 in human GST P1–1 (12Kong K.-H. Takasu K. Inoue H. Takahashi K. Biochem. Biophys. Res. Commun. 1992; 184: 194-197Crossref PubMed Scopus (59) Google Scholar), and Tyr-8 in human GST A1–1 (13Stenberg G. Board P.G. Mannervik B. FEBS Lett. 1991; 293: 153-155Crossref PubMed Scopus (95) Google Scholar)) that has been proposed to activate the bound GSH by stabilizing its thiolate form. The blowfly Lucilia cuprina infests Australian sheep flocks, and the GST isoenzyme purified from this species (14Board P.G. Russel R.J. Marano R.J. Oakeshott J.G. Biochem. J. 1994; 299: 425-430Crossref PubMed Scopus (37) Google Scholar) is possibly involved in the mechanism of insecticide resistance. TheLucilia GST has been classified as a Theta class GST on the basis of its primary structure, but the crystal structure shows the equivalent tyrosine residue within the N-terminal region (Tyr-5) to be 13.9 Å away from the thiol group of GSH (15Wilce M.C.J. Board P.G. Feil S.C. Parker M.W. EMBO J. 1995; 14: 2133-2143Crossref PubMed Scopus (218) Google Scholar). Therefore, other residues could replace Tyr-5 and be involved in the activation of GSH. From the crystal structure, Ser-9 is found to be within hydrogen bond distance of the sulfur atom of GSH (3.9 Å), and the hydroxyl group of Tyr-113 may also form a hydrogen bond with the GSH sulfur atom through a water molecule. This tyrosine residue is not conserved in all of the Theta class isoenzymes, and it is absent in the human Alpha class GST. Data so far obtained on L. cuprina GST show that Tyr-113 is not significantly involved in catalysis (16Board P.G. Coggan M. Wilce M.C.J. Parker M.W. Biochem. J. 1995; 311: 247-250Crossref PubMed Scopus (123) Google Scholar). Nevertheless, an equivalent tyrosine residue plays an important catalytic role in the Mu class GST. X-ray diffraction of GST M1–1 in complex with a transition state analogue shows ς-complex stabilization by hydrogen-bonding interactions with the hydroxyl groups of Tyr-6 and Tyr-115 (17Ji X. Armstrong R.N. Gilliland G.L. Biochemistry. 1993; 32: 12949-12954Crossref PubMed Scopus (89) Google Scholar); moreover, the hydroxyl group of Tyr-115 is involved both in chemical and physical steps of catalysis, and its removal has different effects, depending on which of these steps is rate-limiting (18Johnson W.W. Liu S. Ji X. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1993; 268: 11508-11511Abstract Full Text PDF PubMed Google Scholar). In the GST P1–1, the equivalent Tyr-108 shows a multifunctional role in catalysis (19Lo Bello M. Oakley A.J. Battistoni A. Mazzetti A.P. Nuccetelli M. Mazzarese G. Rossjohn J. Parker M.W. Ricci G. Biochemistry. 1997; 36: 6207-6217Crossref PubMed Scopus (60) Google Scholar); the hydroxyl function of Tyr-108 stabilizes the transition state for the Michael addition of GSH to ethacrynic acid, whereas it has a negative influence when 7-chloro-4-nitrobenzene-2-oxa-1,3-diazole is used as cosubstrate. Mutation of Tyr-108 in Phe yields a 7-fold increase of the turnover number, and the additional link between Tyr-108 and the cosubstrate may increase the internal friction of the protein lowering the k cat (20Caccuri A.M. Ascenzi P. Antonini G. Parker M.W. Oakley A.J. Chiessi E. Nuccetelli M. Battistoni A. Bellizia A. Ricci G. J. Biol. Chem. 1996; 271: 16193-16198Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar). With regard to Ser-9, this residue is conserved in all Theta class isoenzymes, and its mutation to Ala strongly lowers the activity of Lucilia GST (16Board P.G. Coggan M. Wilce M.C.J. Parker M.W. Biochem. J. 1995; 311: 247-250Crossref PubMed Scopus (123) Google Scholar). The role in catalysis of the equivalent Ser-11 in human Theta GSTT2–2 has been recently studied (21Tan K.L. Chelvanayagam G. Parker M.W. Board P.G. Biochem. J. 1996; 319: 315-321Crossref PubMed Scopus (64) Google Scholar); in that case, the contribution of serine varies with the nature of the second substrate. The aim of this paper is to investigate the catalytic mechanism of theLucilia GST and the possible role of Ser-9 and Tyr-113 as suggested by the Lucilia GST crystal structure. L. cuprina GST wild type, Y113F, and S9A mutants were expressed in Escherichia coli and purified as reported by Board et al. (14Board P.G. Russel R.J. Marano R.J. Oakeshott J.G. Biochem. J. 1994; 299: 425-430Crossref PubMed Scopus (37) Google Scholar, 16Board P.G. Coggan M. Wilce M.C.J. Parker M.W. Biochem. J. 1995; 311: 247-250Crossref PubMed Scopus (123) Google Scholar). With regard to the S9A mutant, the enzyme was partially purified on a DE52 ion exchange column, since this mutant loses its activity almost completely after the GSH affinity column. GSH, 1-chloro-2,4-dinitrobenzene (CDNB), and 1-fluoro-2,4-dinitrobenzene (FDNB) were purchased from Sigma. Glycerol was a BDH product. Spectrophotometric measurements were performed with a double beam Uvikon 940 spectrophotometer Kontron equipped with a cuvette holder thermostatted at 25 °C. Steady state kinetics ofLucilia GST with CDNB as cosubstrate were measured at 340 nm as previously reported (22Habig W.H. Jakoby W.B. Methods Enzymol. 1981; 77: 398-405Crossref PubMed Scopus (2118) Google Scholar). Kinetic experiments were carried out in 1 ml (final volume) of 0.1 m potassium phosphate buffer, pH 6.5, containing variable amounts of substrates and 0.2–0.8 μg of enzyme, except for the S9A mutant, where about 40 μg of enzyme was utilized. The reaction rates were measured at 0.1-s intervals for a total period of 12 s. Initial rates were determined by linear regression and corrected for the spontaneous reaction.S 0.5CDNB was obtained at fixed GSH (10 mm) and variable CDNB concentrations (from 20 μm to 2 mm). S0.5GSH was obtained at fixed CDNB (1 mm) and variable GSH concentrations (from 10 μm to 20 mm). Data of v versus[S] were fitted to Equation 1, v=Vmax/(1+K/[S]nH)Equation 1 and the best fit gave V max,S 0.5 (S 0.5 =K 1/n H), and Hill coefficient (n H) for each substrate. The protein concentration was obtained from the protein absorbance at 280 nm assuming an ε1 mg/ml of 1.41 for Theta wild type and 1.36 for the Y113F mutant; extinction coefficients were calculated on the basis of the amino acid sequence as reported by Gill et al. (23Gill S.C. von Hippel P.H. Anal. Biochem. 1989; 182: 319-326Crossref PubMed Scopus (5083) Google Scholar). For the S9A mutant, the absorbance at 280 nm of the enzyme partially purified on a DE52 ion exchange column was corrected for the relative amount of the S9A mutant derived from SDS-polyacrylamide gel electrophoresis analysis. A molecular mass of 23.9 kDa per GST subunit was used in the calculations. The pH dependence ofk cat/ S0.5CDNBwas obtained as reported above by recording the enzymatic reaction in the following buffers (0.1 m): sodium acetate buffer between pH 4.5 and pH 5.5 and potassium phosphate buffer between pH 6.0 and pH 8.7. Control studies showed that the affinity of the enzyme toward GSH does not change in the pH range utilized. The pH dependence of the GS− bound to LuciliaGST was obtained by difference spectroscopy. In a typical experiment, 0.5 mm GSH was added to 12 μm GST active sites in 1 ml of 0.1 m suitable buffer. The amount of thiolate formed at each pH was monitored with the peak-to-trough amplitude between 240 and 300 nm (ε240 nm = 5,000m−1 cm−1) after subtraction of the spectral contributions of free enzyme and of free GSH (in a serum bovine albumin solution showing an absorbance at 240 nm similar to that of GST sample). pK a values were obtained from kinetic and spectroscopic experiments by fitting the data to Equation 2. y=ylim/(1+10pKa−pH)Equation 2 Binding of GSH to LuciliaGST was studied by difference spectroscopy by following the amount of GS− formed in the active site. In a typical experiment, GSH (ranging from 5 μm up to 4 mm) was added to 1 ml containing 25 μm GST active sites in 0.1m potassium-phosphate buffer, pH 6.5. The amount of GS− bound was monitored with the peak-to-trough amplitude between 245 and 300 nm (ε245 nm = 3,800m−1 cm−1) after subtraction of the spectral contributions of free enzyme and of free GSH (in a serum bovine albumin solution showing an absorbance at 245 nm similar to that of GST sample). The 245-nm wavelength was selected to minimize the absorbance of the high amount of free GSH utilized. Data were fitted to Equation 1, where v and V max were substituted by GS− bound and total GST active sites, respectively. The second order kinetic constants at pH 6.5 for the spontaneous reaction of GSH with CDNB and FDNB and the turnover numbers at pH 6.5 forLucilia GST with CDNB and FDNB as cosubstrates were obtained as previously reported (24Caccuri A.M. Ascenzi P. Lo Bello M. Federici G. Battistoni A. Mazzetti P. Ricci G. Biochem. Biophys. Res. Commun. 1994; 200: 1428-1434Crossref PubMed Scopus (13) Google Scholar). Kinetic parameters were obtained at 25 °C by using 0.1 m potassium phosphate buffer, pH 6.5, containing variable glycerol concentrations as already described (25Ricci G. Caccuri A.M. Lo Bello M. Rosato N. Mei G. Nicotra M. Chiessi E. Mazzetti A.P. Federici G. J. Biol. Chem. 1996; 271: 16187-16192Abstract Full Text Full Text PDF PubMed Scopus (76) Google Scholar). Rapid kinetic experiments were performed on an Applied Photophysics kinetic spectrometer stopped-flow instrument equipped with a temperature-regulated observation chamber with a 1-cm light path and 1-ms dead time. In a typical experiment,Lucilia GST (22 μm), in 0.1 mpotassium phosphate buffer, pH 6.5, containing GSH (20 mm), was mixed with CDNB (2 mm) dissolved in the same buffer. The reaction was followed spectrophotometrically at 340 nm and at different temperatures (between 2.2 and 25 °C). The time course of the product (P) formation was fitted to Equation 3, [P]=[E0](k1/(k1+k2))2−[E0](k1/(k1+k2))2exp(−(k1+k2)t) +[E0](k1/(k1+k2))k2tEquation 3 which gives the pseudo-first order rate constant for the formation of the enzyme-bound product (k 1) and the first order rate constant for the release of product from the active site (k 2) (26Fersht A. Enzyme Structure and Mechanism. 2nd Ed. W. H. Freeman and Co., New York1985: 121-154Google Scholar). A well documented property of GSTs is the ability to lower the pK a of the sulfhydryl group of the bound GSH. The acidity constant of GSH in the active site ranges between 6.0 and 6.5 for the Alpha (27Bjornestedt R. Stenberg G. Widersten M. Board P.G. Sinning I. Jones T.A. Mannervik B. J. Mol. Biol. 1995; 247: 765-773Crossref PubMed Scopus (102) Google Scholar), Mu (11Liu S. Zhang P. Ji X. Johnson W.W. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1992; 267: 4296-4299Abstract Full Text PDF PubMed Google Scholar, 28Graminski G.F. Kubo Y. Armstrong R.N. Biochemistry. 1989; 28: 3562-3568Crossref PubMed Scopus (165) Google Scholar), and Pi (12Kong K.-H. Takasu K. Inoue H. Takahashi K. Biochem. Biophys. Res. Commun. 1992; 184: 194-197Crossref PubMed Scopus (59) Google Scholar) class GSTs. Tyr-6 in rat GST M1–1 (11Liu S. Zhang P. Ji X. Johnson W.W. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1992; 267: 4296-4299Abstract Full Text PDF PubMed Google Scholar), Tyr-7 in human GST P1–1 (12Kong K.-H. Takasu K. Inoue H. Takahashi K. Biochem. Biophys. Res. Commun. 1992; 184: 194-197Crossref PubMed Scopus (59) Google Scholar), and Tyr-8 in human GST A1–1 (13Stenberg G. Board P.G. Mannervik B. FEBS Lett. 1991; 293: 153-155Crossref PubMed Scopus (95) Google Scholar) have a crucial role in stabilization of the thiolate form of GSH. Whether Lucilia GST activates the bound GSH in a manner that is similar to the other GST isoenzymes is still an open question. The crystal structure of Theta GST from L. cuprina blowfly (15Wilce M.C.J. Board P.G. Feil S.C. Parker M.W. EMBO J. 1995; 14: 2133-2143Crossref PubMed Scopus (218) Google Scholar) shows the equivalent tyrosine, near the N terminus (Tyr-5), is 13.9 Å away from the thiol group of bound GSH but suggests that two active site residues, either Ser-9 or Tyr-113, may replace Tyr-5 in the GSH activation. Direct evidence for the forced deprotonation of GSH in the active site of Lucilia GST comes from spectroscopic experiments. The differential spectrum of the binary complex GST·GSH, obtained under nonsaturating GSH concentration and at pH 7.0 (Fig.1, inset), shows an absorption band centered at 240 nm that is typical of a thiolate anion. The absorbance value suggests that more than 50% of the bound GSH is deprotonated at neutral pH values. The pH dependence of the thiolate band shows a pK a of 6.6 for the bound GSH (Fig. 1, Table I). The pH dependence ofk cat/ S0.5CDNB, which should reflect a kinetically relevant ionization of the GST·GSH complex, gives an apparent pK a value of 6.5 (Fig.2 and Table I). These spectroscopic and kinetic experiments provide the first evidence that a Theta-like isoenzyme lowers the pK a of the sulfhydryl group of the bound GSH as occurs in other GST classes despite replacement of the essential tyrosine residue in the latter with a serine residue in the former (11Liu S. Zhang P. Ji X. Johnson W.W. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1992; 267: 4296-4299Abstract Full Text PDF PubMed Google Scholar, 12Kong K.-H. Takasu K. Inoue H. Takahashi K. Biochem. Biophys. Res. Commun. 1992; 184: 194-197Crossref PubMed Scopus (59) Google Scholar, 27Bjornestedt R. Stenberg G. Widersten M. Board P.G. Sinning I. Jones T.A. Mannervik B. J. Mol. Biol. 1995; 247: 765-773Crossref PubMed Scopus (102) Google Scholar, 28Graminski G.F. Kubo Y. Armstrong R.N. Biochemistry. 1989; 28: 3562-3568Crossref PubMed Scopus (165) Google Scholar). To check the influence of the proximal Tyr-113 residue, difference spectroscopy experiments were performed on the Y113F mutant. As shown in Fig. 1, the GSH thiolate band is still present, and the pH dependence of the mercaptide absorption band at 240 nm indicates a pK a value of about 6.5, very close to that found with the wild type enzyme (Table I). Again, the pH dependence ofk cat/ S0.5CDNBparallels the spectroscopic data showing a pK a value of 6.3 (Fig. 2 and Table I). Thus, the hydroxyl group of Tyr-113 does not seem to play a role in the forced deprotonation of the bound GSH. Ser-9 seems to be involved; the S9A mutant has been only partially purified, and direct spectroscopic evidence for the ionization of the bound GSH cannot be obtained. However, the pH dependence ofk cat/ S0.5CDNBidentifies an apparent pK a of 7.6, about 1 pH unit higher than that found for the wild type (Fig. 2, inset, and Table I).Table IpH dependence of kinetic and spectroscopic parametersKinetic parametersSpectroscopic parameterspK ak cat/S 0.5limpK a[GS−]/[GST]lims −1 m −1Wild type6.48 ± 0.14(5.3 ± 0.5) × 1056.60 ± 0.110.82 ± 0.07Y113F6.33 ± 0.15(15.3 ± 1.1) × 1056.55 ± 0.080.84 ± 0.05S9A7.57 ± 0.03(17.2 ± 0.3) × 103k cat/S 0.5lim represents the limiting value of the specificity constant at high pH values; [GS−]/[GST]lim represents the limiting value of the relative amount of GSH thiolate at high pH values. Open table in a new tab Figure 2Kinetic evidence for GSH ionization. pH dependence ofk cat/ S0.5CDNBfor the Lucilia GST wild type (○) and Y113F mutant (•) is shown. Inset, pH dependence ofk cat/ S0.5CDNBfor the S9A mutant. Kinetic parameters were obtained at 25 °C under saturating GSH (10 mm) and variable CDNB concentrations as reported under "Experimental Procedures." Each experimental point is the mean of three determinations, and S.E. for each point does not exceed 5%. Lines were obtained by fitting the data to Equation 2.View Large Image Figure ViewerDownload Hi-res image Download (PPT) k cat/S 0.5lim represents the limiting value of the specificity constant at high pH values; [GS−]/[GST]lim represents the limiting value of the relative amount of GSH thiolate at high pH values. Steady state kinetics of Lucilia GST do not strictly obey the Michaelis-Menten equation. When the concentration of GSH or CDNB is varied, at fixed cosubstrate concentrations, double reciprocal plots are concave down versus GSH and concave up versusCDNB (Fig. 3). This behavior may be a signal that the enzyme follows a steady state kinetic mechanism where a preferred pathway for the ternary complex exists (29Segel I.H. Enzyme Kinetics. John Wiley & Sons, Inc., New York1975: 460-461Google Scholar) or, alternatively, may be due to a cooperative binding of the substrates to the enzyme as already reported for the Cys-47 mutants of GST P1–1 (30Ricci G. Lo Bello M. Caccuri A.M. Pastore A. Nuccetelli M. Parker M.W. Federici G. J. Biol. Chem. 1995; 270: 1243-1248Abstract Full Text Full Text PDF PubMed Scopus (92) Google Scholar). To discriminate between these possibilities, the isothermic binding of GSH was studied. Binding of GSH to other GST classes has been easily measured by following the intrinsic fluorescence quenching of the enzyme (31Caccuri A.M. Aceto A. Rosato N. Di Ilio C. Piemonte F. Federici G. Ital. J. Biochem. 1991; 40: 304-311PubMed Google Scholar), but the intrinsic fluorescence of theLucilia GST is not perturbed by the addition of GSH up to a 20 mm concentration. The isothermic binding curve of GSH has been obtained by measuring the amount of GSH thiolate that is formed at the active site as function of the substrate concentration. At pH 6.5, a KDGSH of 107 μm and a Hill coefficient of about 0.90 have been calculated (Table II), indicating a nearly Michaelian behavior. Similar nonhyperbolic kinetics but noncooperative binding of substrates to the enzyme have been already described for the rat GSTs M1–1, M1–2, and A3–3 (32Ivanetich K.M. Goold R.D. Sikakana C.N.T. Biochem. Pharmacol. 1990; 39: 1999-2004Crossref PubMed Scopus (34) Google Scholar, 33Jakobson I. Warholm M. Mannervik B. J. Biol. Chem. 1979; 254: 7085-7089Abstract Full Text PDF PubMed Google Scholar) and explained by a steady state random sequential Bi Bi mechanism. The dissociation constant (K D ) of GSH, obtained from binding experiments, is almost 5 times lower than theS 0.5 value derived from kinetic experiments; this discrepancy may be explained by a negative effect of CDNB on GSH binding, as already observed in GST P1–1 (34Ivanetich K.M. Goold R.D. Biochim. Biophys. Acta. 1989; 998: 7-13Crossref PubMed Scopus (44) Google Scholar), or may be the consequence of a steady state random mechanism in whichS 0.5 does not correspond toK D . Table II contains kinetic and thermodynamic data obtained with Lucilia GST wild type and Y113F and S9A mutants; both mutants show S 0.5 values toward GSH and CDNB and Hill coefficients comparable with that of wild type. On the other hand, the Y113F turnover number is about 3 times higher than that of the wild type, while the S9A mutant shows an extremely low specific activity (about 0.5% of that of wild type).Table IIKinetic and thermodynamic parameters at pH 6.5k catk cat/S 0.5S 0.5CDNBn H 2-an H obtained at constant GSH and variable CDNB concentrations.S 0.5GSHn H 2-bn H obtained at constant CDNB and variable GSH concentrations.K D GSHn H 2-cn H obtained from GSH binding experiments.s −1s −1 m −1μmμmμmWild type53 ± 1(3.2 ± 0.2) × 105165 ± 81.24 ± 0.03530 ± 410.72 ± 0.02107 ± 110.90 ± 0.02Y113F144 ± 2(7.8 ± 0.5) × 105185 ± 101.17 ± 0.01320 ± 200.83 ± 0.02S9A0.28 ± 0.02(1.3 ± 0.1) × 103208 ± 301.10 ± 0.01540 ± 710.74 ± 0.02Kinetic and binding parameters were determined as described under "Experimental Procedures." S 0.5 represents the substrate concentration that yields half of the V maxvalue.2-a n H obtained at constant GSH and variable CDNB concentrations.2-b n H obtained at constant CDNB and variable GSH concentrations.2-c n H obtained from GSH binding experiments. Open table in a new tab Kinetic and binding parameters were determined as described under "Experimental Procedures." S 0.5 represents the substrate concentration that yields half of the V maxvalue. It is well known that the nucleophilic aromatic substitution reactions proceed via a ς-complex intermediate (35Miller J. Eaborn C. Chapman N.B. Reaction Mechanisms in Organic Chemistry. 8. Elsevier Science Publishing Co., Inc., New York1968: 137-179Google Scholar). Substitution of chlorine by the more electronegative fluorine in the CDNB molecule increases about 50-fold the second order rate constant of the spontaneous reaction with GSH, suggesting that the ς-complex formation is rate-limiting (4Armstrong R.N. Chem. Res. Toxicol. 1991; 4: 131-140Crossref PubMed Scopus (326) Google Scholar). The reaction catalyzed by Lucilia GST is insensitive to the nature of the leaving group; the kcatFDNB to kcatCDNB ratio ranges between 1.0 and 1.6 for wild type and Y113F and S9A mutants. Therefore, a physical step rather than a chemical step may be rate-limiting in this enzymatic reaction as previously observed in human GST P1–1 (24Caccuri A.M. Ascenzi P. Lo Bello M. Federici G. Battistoni A. Mazzetti P. Ricci G. Biochem. Biophys. Res. Commun. 1994; 200: 1428-1434Crossref PubMed Scopus (13) Google Scholar) and in rat GST M1–1 (18Johnson W.W. Liu S. Ji X. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1993; 268: 11508-11511Abstract Full Text PDF PubMed Google Scholar). The effect of viscosity on kinetic parameters has been mainly utilized to study the rate-determining step of very efficient enzymes in which the encounter of substrates with the protein is rate-limiting (k cat/K m = 107 to 108 s−1m−1). A decrease of the pseudo-second order rate constant (k cat/K m ) by increasing the medium viscosity should reflect the weight of diffusive events on catalysis (36Blacklow S.C. Raines R.T. Lim W.A. Zamore P.D. Knowles J.R. Biochemistry. 1988; 27: 1158-1167Crossref PubMed Scopus (249) Google Scholar). The reactions catalyzed by Lucilia GST, wild type, and mutants are characterized byk cat/S 0.5 values ≤ 8 × 105 s−1m−1, so this enzyme is far from being a "perfect" catalyst. In this case, the effect of a viscosogen onk cat should indicate that the rate-limiting step of reaction is related to the product release (18Johnson W.W. Liu S. Ji X. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1993; 268: 11508-11511Abstract Full Text PDF PubMed Google Scholar) or to diffusion-controlled structural transitions of the protein (25Ricci G. Caccuri A.M. Lo Bello M. Rosato N. Mei G. Nicotra M. Chiessi E. Mazzetti A.P. Federici G. J. Biol. Chem. 1996; 271: 16187-16192Abstract Full Text Full Text PDF PubMed Scopus (76) Google Scholar). This may occur for Lucilia GST; in fact, a plot of the reciprocal of the relative catalytic constant kcato/k catagainst the relative viscosity (η/ηo) gives a linear dependence with a slope of about 1.0 for Lucilia GST wild type (Fig. 4), 0.9 for Y113F, and 0.8 for S9A mutants. Diffusion-controlled motions of the protein were already reported to modulate the catalysis of other GST isoenzymes, and different physical events were hypothesized to influence the reaction rate: a conformational change of the ternary complex in the human GST P1–1 (25Ricci G. Caccuri A.M. Lo Bello M. Rosato N. Mei G. Nicotra M. Chiessi E. Mazzetti A.P. Federici G. J. Biol. Chem. 1996; 271: 16187-16192Abstract Full Text Full Text PDF PubMed Scopus (76) Google Scholar) and the product release in the rat GST M1–1 (18Johnson W.W. Liu S. Ji X. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1993; 268: 11508-11511Abstract Full Text PDF PubMed Google Scholar). Pre-steady state kinetics ofLucilia GST were studied by stopped-flow experiments. As shown in Fig. 5, the early stage of catalysis displays a well defined burst phase at 340 nm, which indicates that the product accumulates before the steady state attainment. The burst phase is more evident when the temperature is lowered from 25 to 2.2 °C. At 2.2 °C and saturating substrate concentrations, a burst is observed even in the more active Y113F mutant (Fig. 5 C). Enzyme concentrations, calculated on the basis of the burst amplitude (π) (9.9 and 9.5 μm for the Y113F mutant and wild type, respectively), are close to the protein concentration used in these experiments (11 μm). The reaction catalyzed by the Lucilia GST may be roughly subdivided into two steps: the formation of the enzyme-bound GSH conjugate, governed by the pseudo-first order rate constant (k 1), which summarizes all steps from the substrate binding to the product formation, and the release of product from the active site governed by the first order rate constant (k 2), shown in Scheme I. E+GSH+CDNB→k1EP→k2E+PScheme I The rate constants k 1 andk 2 obtained from pre-steady state kinetics performed at different temperatures are reported in TableIII. The free energy levels of the catalytic steps related to the rate constants k 1and k 2 may be estimated by their temperature dependence. The Arrhenius plot gives activation energy values of about 54 and 104 kJ/mol for k 1 andk 2, respectively (Fig.6), indicating that two very different energy barriers are involved. Temperature dependence ofk cat gives an activation energy value of about 96 kJ/mol, confirming that the release of product is rate-limiting. The rate constant k 2 is much more sensitive to the temperature than k 1, so the gap between the two rate constants becomes smaller as the temperature increases. Pre-steady state data suggest that the hydroxyl group of Tyr-113, which resides in α-helix 4 (15Wilce M.C.J. Board P.G. Feil S.C. Parker M.W. EMBO J. 1995; 14: 2133-2143Crossref PubMed Scopus (218) Google Scholar), affects negatively both rate constantsk 1 and k 2; at 2.2 °C, the Y113F mutant shows a k 1 value of about 325 s−1 and a k 2 of about 18 s−1, while at the same temperature, the wild type shows ak 1 value of about 67 s−1 and ak 2 of about 5 s−1. This may be the consequence of additional interactions involving the hydroxyl group of Tyr-113, which will be discussed below.Table IIITemperature dependence of kinetic parametersTemperatureWild typeY113Fk 1k 2k 1k 2°Cs −1s −1s −1s −12.2675325185.61221415.22934824.7415172The values of the rate constants k 1 andk 2 (see Scheme I) at different temperatures were calculated from the best fit of experimental data according to Equation3. S.E. does not exceed 5%. In the case of the Y113F mutant, it was impossible to find a unique solution to discriminate betweenk 1 and k 2 at a temperature higher than 2.2 °C. Open table in a new tab Figure 6Temperature effect on kinetic parameters. The rate of product accumulating at the active sitek 1 (s−1) (▪), the rate of product release k 2 (s−1) (•), andk cat (s−1) (○) were obtained, between 2.2 and 25 °C, from the best fit of the pre-steady state kinetic data according to Equation 3; for details see "Experimental Procedures." Activation energies were obtained from linear regression of data.View Large Image Figure ViewerDownload Hi-res image Download (PPT) The values of the rate constants k 1 andk 2 (see Scheme I) at different temperatures were calculated from the best fit of experimental data according to Equation3. S.E. does not exceed 5%. In the case of the Y113F mutant, it was impossible to find a unique solution to discriminate betweenk 1 and k 2 at a temperature higher than 2.2 °C. Kinetic and binding experiments show, for the first time in a Theta GST, that the isoenzyme purified from the sheep blowfly L. cuprina is able to lower the pK a of bound GSH about 3 pH units despite the absence, in the G-site, of the crucial tyrosine found in the Alpha, Pi, and Mu class GSTs (11Liu S. Zhang P. Ji X. Johnson W.W. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1992; 267: 4296-4299Abstract Full Text PDF PubMed Google Scholar, 12Kong K.-H. Takasu K. Inoue H. Takahashi K. Biochem. Biophys. Res. Commun. 1992; 184: 194-197Crossref PubMed Scopus (59) Google Scholar, 13Stenberg G. Board P.G. Mannervik B. FEBS Lett. 1991; 293: 153-155Crossref PubMed Scopus (95) Google Scholar). The role played by the hydroxyl group of Ser-9 and also of Tyr-113, two residues possibly involved in the GSH activation, has been examined. Removal of the hydroxyl groups of Tyr-113 and of Ser-9 does not affect the affinity toward both GSH and CDNB. Moreover, the pK a of the E·GSH complex is unchanged in the Y113F mutant, while it shifts from 6.5 to about 7.6 in the S9A mutant, a value still lower than the pK a (9.0) of the GSH in aqueous solution. Another contributor to the lower than expected pK a might be the influence of a helix dipole, since the sulfur atom of GSH lies directly above the N terminus of α-helix 1 (15Wilce M.C.J. Board P.G. Feil S.C. Parker M.W. EMBO J. 1995; 14: 2133-2143Crossref PubMed Scopus (218) Google Scholar). On the other hand, removal of the hydroxyl group of Ser-9, hydrogen-bonded to the thiol group of GSH, results in a very low turnover number (about 0.5% of that of wild type). Similarly, replacement of Tyr-6 with phenylalanine in the rat GST M1–1 (11Liu S. Zhang P. Ji X. Johnson W.W. Gilliland G.L. Armstrong R.N. J. Biol. Chem. 1992; 267: 4296-4299Abstract Full Text PDF PubMed Google Scholar) resulted in a very low turnover number and the pH dependence ofk cat/ KmCDNBfor the E·GSH complex gave a pK a value of 7.8. Zheng and Ornstein (37Zheng Y.-J. Ornstein R.L. J. Am. Chem. Soc. 1997; 119: 1523-1528Crossref Scopus (25) Google Scholar), by using ab initiomolecular orbital calculations, found a pK a value of 7.8 for the bound GSH when the active site tyrosine was replaced by phenylalanine. These results confirm that Ser-9, in L. cuprina GST, is equivalent to the conserved tyrosine residues in the G-site of other GST classes. Like Tyr-6, in the rat GST M1–1, Ser-9 may contribute to the stabilization of the GSH thiolate; however, on the basis of the pK a value of 7.6 in the S9A mutant, a k cat 6-fold lower than that of wild type is expected at pH 6.5, while a 200-fold decrease ofk cat is actually observed. The dramatic lowering of k cat observed in the absence of Ser-9 can be explained neither on the basis of an increased pK a of the bound GSH nor by a different thermal stability between S9A and wild type (data not shown); thus, Ser-9 seems to be mainly implicated in the correct orientation of the sulfhydryl group of GSH in the catalytic step or in the transition state stabilization. In the last few years, a great effort has been made to clarify whether the crucial tyrosine residue acts as a general base proton acceptor or as a hydrogen bond donor in the GSH activation mechanism; in the former, a relevant amount of tyrosinate is required. The functional equivalence between serine and tyrosine suggests that the tyrosinate ion idea may not be physiologically important. In fact, in the LuciliaGST the ionization of the key hydroxyl residue Ser-9 is not possible, and this ionization may be also not be required for the equivalent tyrosine residue in other GST classes. Recently, a consensus pattern for Theta class GSTs based on Lucilia GST structure has been derived and utilized to identify key residues in the polypeptide fold (38Rossjohn J. Board P.G. Parker M.W. Wilce M.C.J. Protein Eng. 1996; 9: 327-332Crossref PubMed Scopus (30) Google Scholar); in this study the serine (or sometimes threonine) residue near the N terminus is found in virtually all Theta class GSTs, so the present findings are probably applicable to varying degrees to all of the family members.