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Visual DNA - Identification of DNA sequence variations by bead trapping

2007; Elsevier BV; Volume: 90; Issue: 6 Linguagem: Inglês

10.1016/j.ygeno.2007.07.014

1089-8646

Patrik L. Ståhl, Jesper Gantelius, Christian Natanaelsson, Afshin Ahmadian, Helene Andersson Svahn, Joakim Lundeberg,

Gene expression and cancer classification

In this paper we describe a method that uses the nearly covalent strength biotin–streptavidin interaction to attach a paramagnetic bead of micrometer size to a DNA molecule of nanometer size, scaling up the spatial size of a query DNA strand by a factor of 1000, making it visible to the human eye. The use of magnetic principles enables rapid binding and washing of detector beads, facilitating a readout of amplified DNA sequences in a few minutes. Here we exemplify the method on mitochondrial DNA variations using an array platform. Visual identification and documentation can be performed with an ordinary mobile phone equipped with a built-in camera.