Simple Assay Method for Susceptibility of Human Lymphocytes to Epstein-Barr Virus Infection
1982; Karger Publishers; Volume: 18; Issue: 1-2 Linguagem: Inglês
10.1159/000149308
ISSN1423-0100
Autores Tópico(s)T-cell and Retrovirus Studies
ResumoOn the basis of the sequential early events induced by Epstein-Barr virus (EBV), the infectability of human lymphocytes to EBV was quantified as a plateau value of the maximum nuclear antigen (EBNA) synthesis preceding cellular DNA synthesis. The frequency of EBV-infectable cells was an average of 7.6% of unfractionated umbilical cord lymphocytes and was 4.9% in the counterparts from human adults. In the B-cell-enriched populations, the average plateau values were 29.2% in umbilical cord and 15.5% in adult samples. This method is simple and applicable for quantitative assay of the sensitivity to EBV infection of a given lymphocyte preparation.
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