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BIBTEX RIS

Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella

2004; American Society for Microbiology; Volume: 42; Issue: 5 Linguagem: Inglês

10.1128/jcm.42.5.2261-2263.2004

ISSN

1098-660X

Autores

Tatiana Bogdanovich, Mikael Skurnik, Peter Stephensen Lübeck, Peter Ahrens, Jeffrey Hoorfar,

Tópico(s)

Escherichia coli research studies

Resumo

ABSTRACT A real-time, genus-specific 5′ nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains ( n = 23) and did not detect non- Brucella strains ( n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.

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