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DETECTION OF MICROMETASTATIC PROSTATE CANCER CELLS IN THE LYMPH NODES BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION IS PREDICTIVE OF BIOCHEMICAL RECURRENCE IN PATHOLOGICAL STAGE T2 PROSTATE CANCER.

2000; Lippincott Williams & Wilkins; Volume: 163; Issue: 4 Linguagem: Inglês

10.1016/s0022-5347(05)67718-x

1527-3792

Takatsugu Okegawa, Kikuo Nutahara, Eiji Higashihara,

Molecular Biology Techniques and Applications

No AccessJournal of UrologyCLINICAL UROLOGY: Original Articles1 Apr 2000DETECTION OF MICROMETASTATIC PROSTATE CANCER CELLS IN THE LYMPH NODES BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION IS PREDICTIVE OF BIOCHEMICAL RECURRENCE IN PATHOLOGICAL STAGE T2 PROSTATE CANCER. Takatsugu Okegawa, Kikuo Nutahara, and Eiji Higashihara Takatsugu OkegawaTakatsugu Okegawa , Kikuo NutaharaKikuo Nutahara , and Eiji HigashiharaEiji Higashihara View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)67718-XAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We evaluated whether detecting prostate cancer cells by the nested reverse transcriptase-polymerase chain reaction (RT-PCR) in lymph nodes has predictive value for serum prostate specific antigen (PSA) recurrence in patients undergoing radical prostatectomy. Materials and Methods: We assessed the presence of prostate cancer cells by RT-PCR for prostate specific membrane antigen and PSA assay in lymph nodes dissected from 38 patients with localized prostate cancer treated with radical prostatectomy. The results of nested RT-PCR assay were compared with biochemical recurrence. Results: Nested RT-PCR was positive in the lymph nodes of 2 of 18 patients (11%) with stage pT2a and 5 of 20 (25%) with stage pT2b disease. All 7 patients had biochemical recurrence. We noted a significant difference in the Kaplan-Meier recurrence-free actuarial probability curve in those with positive and negative nested RT-PCR results for prostate specific membrane antigen, PSA and prostate specific membrane antigen-PSA in the lymph nodes (p = 3.02×10−7, 2.23×10−7 and 3.02×10−7, respectively). Multivariate analysis of serum PSA, Gleason score and preoperative RT-PCR assay in peripheral blood showed that nested RT-PCR for prostate specific membrane antigen, PSA and prostate specific membrane antigen-PSA in the lymph nodes were independent predictors of recurrence (p = 0.0089, 0.0075 and 0.0089, respectively). Conclusions: Nested RT-PCR of the lymph nodes may be a useful pretreatment prognostic test for patients undergoing radical prostatectomy. Further research is necessary using a much larger number of patients with a longer followup. References 1 : 5-Year tumor recurrence rates after anatomical radical prostatectomy for prostate cancer. J Urol, part 21994; 152: 1837. Abstract, Google Scholar 2 : Prediction of progression following radical prostatectomy. A multivariate analysis of 721 men with long-term follow-up. Am J Surg Pathol1996; 20: 286. 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Urology1999; 53: 714. Google Scholar From the Department of Urology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan© 2000 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byOKEGAWA T, KINJO M, NUTAHARA K and HIGASHIHARA E (2018) VALUE OF REVERSE TRANSCRIPTION POLYMERASE CHAIN ASSAY IN PERIPHERAL BLOOD OF PATIENTS WITH UROTHELIAL CANCERJournal of Urology, VOL. 171, NO. 4, (1461-1466), Online publication date: 1-Apr-2004.MENG M and CARROLL P (2018) WHEN IS PELVIC LYMPH NODE DISSECTION NECESSARY BEFORE RADICAL PROSTATECTOMY? A DECISION ANALYSISJournal of Urology, VOL. 164, NO. 4, (1235-1240), Online publication date: 1-Oct-2000. Volume 163Issue 4April 2000Page: 1183-1188 Advertisement Copyright & Permissions© 2000 by American Urological Association, Inc.Keywordsprostateprostate-specific membrane antigenpolymerase chain reactionprostate-specific antigenbiochemical recurrenceMetricsAuthor Information Takatsugu Okegawa More articles by this author Kikuo Nutahara More articles by this author Eiji Higashihara More articles by this author Expand All Advertisement PDF downloadLoading ...