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H‐2‐RESTRICTED RECOGNITION OF MINOR HISTOCOMPATIBILITY ANTIGENS IN DELAYED TYPE HYPERSENSITIVITY

1980; Wiley; Volume: 7; Issue: 4 Linguagem: Inglês

10.1111/j.1744-313x.1980.tb00725.x

1744-313X

Theodorus van der Kwast,

Immunotherapy and Immune Responses

Summary Subcutaneous (s.c.) immunization of mice with viable allogeneic H‐2 compatible spleen cells can induce a persistent state of delayed type hypersensitivity (DTH) to minor histocompatibility (H) antigens which can be evaluated with a footpad swelling assay. The importance of H‐2 compatibility of the injected spleen cells with the recipient for (1) the elicitation of the DTH‐reaction, (2) the induction of DTH‐related effector and memory T cells and (3) the activation of T memory cells was examined with congenic mouse strains. Spleen cells sharing either the K or D region of the H‐2 complex with the recipient could elicit strong DTH‐reactions to minor H antigens, though somewhat less than did fully H‐2 compatible allogeneic spleen cells. H‐2 incompatible cells or cells only sharing I‐region coded antigens elicited relatively weak, though significant, DTH‐reactivity to the minor H antigens. Similar H‐2 requirements for recipient mice were demonstrated in the immune lymphocyte transfer assay. Optimal induction of primary DTH and DTH‐related T memory cells for minor H antigens also required H‐2 identity of the immunizing cells and the recipient. To some extent, H‐2 incompatible cells were able to induce primary DTH‐reactivity and memory for minor H‐antigens. The secondary DTH‐reactivity was not or slightly dependent on the H‐2 haplotype of the cells used for booster injection. It is concluded that the DTH‐related effector cells are restricted in their recongnition of minor H antigens by K‐ or D‐region‐coded antigens. Presumably, macrophage processing of the allogeneic spleen cells after primary and secondary immunization accounts for the capacity of the minor H antigens to activate unprimed T cells and memory T cells when these antigens are presented on H‐2 incompatible cells.