Transcriptional activity and constitutive nuclear localization of the ETS protein Elf‐1
1997; Wiley; Volume: 408; Issue: 1 Linguagem: Inglês
10.1016/s0014-5793(97)00387-6
ISSN1873-3468
AutoresIsa Bredemeier-Ernst, Alfred Nordheim, Ralf Janknecht,
Tópico(s)Immunotherapy and Immune Responses
ResumoElf-1 is a lymphoid-specific transcription factor that belongs to the ETS protein family. It can bind to DNA target sequences within a variety of cytokine genes. We demonstrate that Elf-1 is constitutively localized in the nucleus which is dependent on the presence of amino acids 86-265. Analysis of Gal4-Elf-1 fusion proteins revealed that the N-terminal 86 amino acids of Elf-1 contain a transcriptional activation domain, the activity of which is attenuated by an internal repression domain. Furthermore, Elf-1 interacts specifically with the E74 target sequence and can stimulate transcription driven by the E74 site independent of mitogenic signaling. Thus, Elf-1 is able to stimulate gene transcription which may be required for the development and activity of lymphocytes.
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