First Report of Tomato chlorotic spot virus on Annual Vinca ( Catharanthus roseus ) in the United States
2015; American Phytopathological Society; Volume: 99; Issue: 6 Linguagem: Inglês
10.1094/pdis-12-14-1269-pdn
ISSN1943-7692
AutoresColleen Warfield, Karen E. Clemens, Scott Adkins,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 99, No. 6First Report of Tomato chlorotic spot virus on Annual Vinca (Catharanthus roseus) in the United States PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tomato chlorotic spot virus on Annual Vinca (Catharanthus roseus) in the United StatesC. Y. Warfield, K. Clemens, and S. AdkinsC. Y. WarfieldSearch for more papers by this author, K. ClemensSearch for more papers by this author, and S. AdkinsSearch for more papers by this authorAffiliationsAuthors and Affiliations C. Y. Warfield K. Clemens , Ball Horticultural Company, West Chicago, IL 60185 S. Adkins , USDA-ARS, Fort Pierce, FL 34945. Published Online:14 May 2015https://doi.org/10.1094/PDIS-12-14-1269-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In July 2014, six annual vinca (also known as Madagascar periwinkle) plants with virus-like symptoms (10 to 23% affected for each of six cultivars) grown in a Miami-Dade County, Florida trial garden were submitted to the Plant Pathology Services Lab at Ball Horticultural Company. Symptoms included dark brown stem lesions, chlorotic leaves with brown line patterns, spotting, ringspots, and black petioles. Four additional plants with similar viral symptoms were submitted in August 2014 from the same trial garden. Each of the 10 plants reacted with Tomato spotted wilt virus (TSWV) ImmunoStrip and/or DAS-ELISA reagents (Agdia, Inc., Elkhart, IN) indicating the presence of one or more tospoviruses. Twig blight caused by Colletotrichum truncatum (3 of 4 plants) and aerial blight caused by Phytophthora nicotianae (1 of 4 plants) were also present on the August samples. With the recent identification of Tomato chlorotic spot virus (TCSV) in Florida (Londoño et al. 2012; Webster et al. 2015) and known cross reaction of TSWV serological reagents with closely related tospoviruses, plants were tested for TCSV, TSWV, and Groundnut ringspot virus (GRSV) by reverse transcription (RT)-PCR as previously described (Webster et al. 2013). Total RNA was extracted from representative symptomatic leaves of one plant using RNeasy Plant Mini Kit (Qiagen, Valencia, CA) and tested by RT-PCR with TCSV-specific nucleocapsid (N; TCSV-Nv2/TCSV-Nvc2), glycoprotein precursor (GNGC; GLY3-v/GLY3-vc), or RNA-dependent RNA polymerase (L; TCSV-3′Lv/TCSV-3′Lvc) gene primers (Webster et al. 2011, 2013). Amplicons of the expected sizes were produced with all three TCSV primer sets, whereas primers specific for the N gene of TSWV or GRSV (Webster et al. 2013) did not amplify products. Two of the TCSV amplicons (N and GNGC) were gel-purified and cloned (pGEM-T, Promega, Madison, WI). Six clones of each amplicon were sequenced in both directions and consensus sequences were deposited in GenBank (KP172478 to KP172479). Sequences of both genes showed greater than 96% nucleotide identity with all TCSV isolates in GenBank, including 99 to 100% nucleotide identity with previously characterized TCSV isolates from tomato, pepper, and jimsonweed in Puerto Rico and Florida. In October 2014, three more plants in the same trial garden were submitted for testing. Total RNA was extracted from symptomatic leaves of each plant and tested by RT-PCR with a second set of TCSV-specific N gene primers (Baysal-Gurel et al. 2015). An amplicon of the expected size was produced from all samples. One amplicon was gel-purified and direct-sequenced using the same primers. The N gene sequence from this October 2014 sample (KP172480) was >99% identical to the corresponding portion of the N gene sequence from the August 2014 sample. C. truncatum was again present (2 of 3 plants). To the best of our knowledge, this is the first report from any location of TCSV infection of annual vinca, a widely-grown bedding plant. Host range expansion of TCSV into flowering ornamental crops opens additional avenues for spread of this emerging tospovirus.References:Baysal-Gurel, F., et al. 2015. Plant Dis. 99:163. https://doi.org/10.1094/PDIS-06-14-0639-PDN Link, ISI, Google ScholarLondoño, A., et al. 2012. Trop. Plant Pathol. 37:333. https://doi.org/10.1590/S1982-56762012005000001 Crossref, ISI, Google ScholarWebster, C. G., et al. 2011. Virology 413:216. https://doi.org/10.1016/j.virol.2011.02.011 Crossref, ISI, Google ScholarWebster, C. G., et al. 2013. Plant Health Prog. Online. https://doi.org/10.1094/PHP-2013-0812-01-BR. Google ScholarWebster, C. G., et al. 2015. Phytopathology 105:388. Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 6 June 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 23 Jun 2015Published: 14 May 2015First Look: 13 Jan 2015Accepted: 8 Jan 2015 Page: 895 Information© 2015 The American Phytopathological SocietyCited byThe role of ornamental plants as hosts of Tomato chlorotic spot virus and its vector thrips affecting tomato production12 March 2023 | Entomologia Experimentalis et Applicata, Vol. 16Colletotrichum truncatum (soyabean anthracnose)CABI Compendium, Vol. CABI CompendiumPhytophthora nicotianae (black shank)CABI Compendium, Vol. CABI CompendiumTomato chlorotic spot virusCABI Compendium, Vol. CABI CompendiumField distribution and disease incidence of tomato chlorotic spot virus, an emerging virus threatening tomato production in South Florida13 August 2019 | Tropical Plant Pathology, Vol. 44, No. 5Field Evaluation of Tomato Cultivars for Tolerance to Tomato Chlorotic Spot TospovirusShouan Zhang, Xiaohui Fan, Yuqing Fu, Qingren Wang, Eugene McAvoy, and Dakshina R. 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