Simultaneous microchemical analysis of glycerol, fatty acid, and phosphorus in complex lipids
1971; Elsevier BV; Volume: 16; Issue: 3 Linguagem: Inglês
10.1016/0026-265x(71)90029-4
ISSN1095-9149
AutoresDeWayne Townsend, Brian P. Livermore, H. M. Jenkin,
Tópico(s)Advanced Proteomics Techniques and Applications
ResumoAbstract A single, small glycerol-containing lipid sample (50–200 nmoles) after a mild hydrolysis could be used for analysis of fatty acids, glycerol, and phosphorus. The range of the assays was from 10–500 nmoles for each lipid. A key to success in the analysis was using highly purified, freshly distilled reagents to reduce the background. A charcoal adsorption of the glycerol color reagent was particularly important. The usefulness and high reproducibility of this technique has been demonstrated in analysis of small quantities of lipids from tissue culture cells, plants, and bacteria. The structure and purity of lipid samples can be further verified by obtaining the expected molar ratios of three structural moieties of complex lipids.
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