General concepts for PCR primer design.
1993; Cold Spring Harbor Laboratory Press; Volume: 3; Issue: 3 Linguagem: Inglês
10.1101/gr.3.3.s30
ISSN1549-5469
AutoresCarl W. Dieffenbach, Todd M. Lowe, Gabriela Dveksler,
Tópico(s)Bacteriophages and microbial interactions
ResumoPCR is a technology born of the modern molecular biology era.The enzyme PARAMETERS USED IN BASIC PCR PRIMER DESIGNPrimer design is aimed at obtaining a balance between two goals: specificity and efficiency of amplification.Specificity is defined as the frequency with which a mispriming event occurs.Primers with mediocre to poor specificity tend to produce PCR products with extra unrelated and undesirable ampli-$30 PCR Methods and Applications 3:$30-S37 9 by Cold Spring Harbor Laboratory
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