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Rapid liquid–liquid extraction of cocaine from urine for gas chromatographic–mass spectrometric analysis

1997; Elsevier BV; Volume: 692; Issue: 1 Linguagem: Inglês

10.1016/s0378-4347(96)00459-8

1872-812X

Diana Garside, Bruce A. Goldberger, Kenzie L. Preston, Edward J. Cone,

Psychedelics and Drug Studies

A novel, simple and economic liquid-liquid extraction method for isolating cocaine from urine was developed utilizing gas chromatography-mass spectrometry (GC-MS) for analysis and quantification. The use of a single nonpolar organic solvent allowed only nonpolar analytes to be extracted from the biological fluid, and consequently, no derivatization step was necessary before GC-MS analysis. Large numbers of specimens (>60) can be extracted in approximately 3 h with this procedure. The method is highly precise (C.V. 98%), sensitive (limit of detection of 5 ng/ml) and has a mean recovery of 48.8%.