Portal de Periódicos da CAPES

The contribution of pH‐dependent mechanisms to fatigue at different intensities in mammalian single muscle fibres

1998; Wiley; Volume: 512; Issue: 3 Linguagem: Inglês

10.1111/j.1469-7793.1998.831bd.x

1469-7793

Eva R. Chin, David G. Allen,

Sports Performance and Training

The contribution of intracellular pH (pH i ) to the failure of Ca 2+ release and inhibition of contractile proteins observed during fatigue was assessed in single intact mouse muscle fibres at 22 °C. Fatigue was induced by repeated tetani at intensities designed to induce different levels of intracellular acidosis. Force and either intracellular free Ca 2+ concentration ([Ca 2+ ] i ; measured using indo‐1) or pH i (measured using SNARF‐1) were recorded in fibres fatigued at two different intensities. Intensity was varied by the repetition rate of tetani and quantified by the duty cycle (the fraction of time when the muscle was tetanized). Stimulation at the low intensity (duty cycle ∼0.1) reduced force to 30 % of initial values in 206 ± 21 s (60 ± 7 tetani); at the high intensity (duty cycle ∼0.3) force was reduced to 30 % in 42 ± 7 s (43 ± 7 tetani) ( P < 0.05 ; n = 14 ). When force was reduced to 30 % of initial values, tetanic [Ca 2+ ] i had fallen from 648 ± 87 to 336 ± 64 n m (48 % decrease) at the low intensity but had only fallen from 722 ± 84 to 468 ± 60 n m (35 % decrease) at the higher intensity ( P < 0.05 low vs. high intensity; n = 7 ). Fatigue resulted in reductions in Ca 2+ sensitivity of the contractile proteins which were greater at the high intensity (pre‐fatigue [Ca 2+ ] i required for 50 % of maximum force (Ca 50 ) = 354 ± 23 n m ; post‐fatigue Ca 50 = 421 ± 48 n m and 524 ± 43 n m for low and high intensities, respectively). Reductions in maximum Ca 2+ ‐activated force ( F max ) were similar at the two intensities (pre‐fatigue F max = 328 ± 22 μN; post‐fatigue F max = 271 ± 20 and 265 ± 19 μN for low and high intensities, respectively). Resting pH i was 7.15 ± 0.05. During fatigue at the low intensity, pH i was reduced by 0.12 ± 0.02 pH units and at the high intensity pH i was reduced by 0.34 ± 0.07 pH units ( P < 0.05 ; n = 5 ). Our results indicate that the more rapid fall in force at a high intensity is due to a reduction in Ca 2+ sensitivity of the contractile proteins, probably related to the greater acidosis. Our data also indicate that the failure of Ca 2+ release and reduced maximum Ca 2+ ‐activated force observed during fatigue are not due to reductions in intracellular pH.