The complete cDNA and deduced amino acid sequence of equine IgE
1995; Elsevier BV; Volume: 32; Issue: 1 Linguagem: Inglês
10.1016/0161-5890(94)00143-o
ISSN1872-9142
AutoresPedro Navarro, Dina P. Barbis, Douglas F. Antczak, J.E. Butler,
Tópico(s)T-cell and B-cell Immunology
ResumoThe cDNA from a transcript encoding the complete heavy chain of the equine immunoglobulin IgE has been cloned and sequenced. A fragment of the equine epsilon gene was amplified from cDNA using PCR and this fragment was then used to probe a horse cDNA library prepared from peripheral blood lymphocytes. A recombinant clone containing the cDNA encoding the complete horse epsilon chain and its associated V-D-J and leader, was subsequently isolated and sequenced. Comparison of the deduced amino acid sequence of equine IgE with the C epsilon heavy chains of other species indicates it to be most similar to human C epsilon (54%), followed by sheep C epsilon (52%); the greatest sequence similarity was found in the C epsilon 3 and C epsilon 4 domains among the species compared. The associated V-region had greatest similarity in FR1 to those reported for sheep and the cow (> 80%) but less than 60% to the VH sequences of other species in FR3.
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