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Oxygen Diffusion through Natural Extracellular Matrices: Implications for Estimating “Critical Thickness” Values in Tendon Tissue Engineering

2008; Mary Ann Liebert, Inc.; Volume: 14; Issue: 4 Linguagem: Inglês

10.1089/tea.2006.0361

1937-335X

Caroline Androjna, Jorge E. Gatica, Joanne Belovich, Kathleen A. Derwin,

3D Printing in Biomedical Research

Oxygen is necessary for maintaining cell proliferation and viability and extracellular matrix (ECM) production in 3-dimensional tissue engineering. Typically, diffusion is the primary mode for oxygen transport in vitro; thus, ensuring an adequate oxygen supply is essential. In this study, we determined the oxygen diffusion coefficients of 3 natural ECMs that are being investigated as construct scaffolds for tendon tissue engineering: small-intestine submucosa (SIS), human dermis (Alloderm), and canine fascia lata. Diffusion coefficients were determined using a standard diffusion cell system. The ranges for each matrix type were: SIS: 7 x 10(-6) - 2 x 10(-5) cm2/s, Alloderm: 1.9 - 3.1 x 10(-5) cm2/s, and canine fascia lata: 1.6 - 4 x 10(-5) cm2/s. We used the experimental oxygen diffusivity data for these natural ECMs in a mathematical model of oxygen diffusion through a cell-seeded scaffold to estimate the critical size of cell-seeded scaffold that can be cultured in vitro.