Artigo Acesso aberto

Purification of Ferredoxin-NADP + Reductase, Flavodoxin and Ferredoxin from a Single Batch of the Cyanobacterium Anabaena PCC 7119

1991; Marcel Dekker; Volume: 21; Issue: 4 Linguagem: Inglês

10.1080/10826069108018571

ISSN

2331-0510

Autores

José J. Pueyo, Carlos Gómez‐Moreno,

Tópico(s)

Algal biology and biofuel production

Resumo

Abstract Methods are described for the simultaneous isolation of ferredoxin-NADP+ reductase, ferredoxin and flavodoxin from large quantities of the cyanobacterium Anabaena PCC 7119 allowing the use of a single batch of cells. The ultraviolet-visible spectra and the extinction coefficients of ferredoxin-NADP+ reductase and ferredoxin were determined. The purification procedure also yields enriched fractions of phycobiliproteins and cytochrome c 553.

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