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First Report of Rose cryptic virus 1 in Rosa Plants in Canada

2014; American Phytopathological Society; Volume: 99; Issue: 4 Linguagem: Inglês

10.1094/pdis-09-14-0921-pdn

1943-7692

D. James, James Phelan, Á. Varga, M. Rott, Jean A. Bérubé,

Plant-Microbe Interactions and Immunity

HomePlant DiseaseVol. 99, No. 4First Report of Rose cryptic virus 1 in Rosa Plants in Canada PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Rose cryptic virus 1 in Rosa Plants in CanadaD. James, J. Phelan, A. Varga, M. Rott, and J. A. BerubeD. JamesSearch for more papers by this author, J. PhelanSearch for more papers by this author, A. VargaSearch for more papers by this author, M. RottSearch for more papers by this author, and J. A. BerubeSearch for more papers by this authorAffiliationsAuthors and Affiliations D. James J. Phelan A. Varga M. Rott , Canadian Food Inspection Agency, Sidney Laboratory, North Saanich, BC, Canada, V8L 1H3 J. A. Berube , Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, CP 10380 Succ Ste-Foy, Quebec, Canada, G1V 4C7. Published Online:1 Apr 2015https://doi.org/10.1094/PDIS-09-14-0921-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Rose cryptic virus 1 (RCV-1; 3) also known as Rosa multiflora cryptic virus (RMCV; 1,4), is thought to be a tripartite double-stranded (ds) RNA virus, with dsRNA 1 encoding the putative RNA-dependent RNA polymerase (RdRp) (3), and dsRNA 2 and 3 described as encoding proteins of unknown functions (4), or probably proteins associated with the viral capsid (3). The virus has been detected in symptomless roses (3) and in rose plants displaying spring dwarf symptoms (4) or symptoms of rose rosette (1). Martin and Tzanetakis (1) indicated that RCV-1 may possibly play a role if a virus complex is associated with these diseases. In a study to identify potential pathways for the international movement of RNA viruses, several imported and local plant genera were screened by next-generation-sequencing (NGS) analysis of dsRNA extracts, using the Illumina HiSeq 2000 platform. Plants screened in 2012 and 2013 included ornamental Rosa cvs. Antique 89, Carefree Sprit, Chateau Merlot, Flamingo Kolorscape, Goldener Olymp, Lydia, Magic Meidiland, Phyllis Bide, Pink Flamingo, and What a Peach. RCV-1 was detected in dsRNA extracts from locally purchased Rosa cvs. Goldener Olymp and What a Peach. RCV-1-specific RT-PCR, which targets dsRNA 1 (4), was used to confirm the presence of the virus. Amplified fragments of the expected size (359 bp) were observed, with nucleotide sequence identities of 99 to 100% to the corresponding dsRNA 1 sequences of RCV-1 (GenBank EU413666; EU024675). Sanger-sequencing, single-primer amplification technique (SPAT), and rapid amplification of cDNA ends (RACE) were used to assist in generating complete sequences, and to confirm the terminal sequences, of the three segments of the Goldener Olymp (GO) isolate of RCV-1. The full-length sequences of dsRNA 1, dsRNA 2, and dsRNA 3 of RCV-1 isolate GO were determined to consist of 1,748, 1,483, and 1,447 nt, respectively. The sequences, both for the nt and the deduced amino acids, are 99 to 100% identical to the corresponding sequences of RCV-1, accessions EU413666, EU413667, and EU413668, respectively (1). Similar levels of identity were observed in the corresponding genome segments with complete sequences described by Salem et al (4). RCV-1 is closely related to members of the genus Partitivirus (3), and it is likely a member of the family Partitiviridae (4). Members of the genus Partitivirus usually consist of a bipartite genome (2). It has been suggested that the tripartite genome associated with RCV-1 may represent co-infection by two different partitiviruses; the virus may possess two different versions of dsRNA2; or the third dsRNA element may represent a satellite virus (2). In this deep sequencing study, no evidence of the RdRp (dsRNA 1) of a second virus was detected. It is indeed possible that dsRNA 2 and dsRNA 3 encode functionally different proteins representing a new group within the family Partitiviridae. This is the first report describing the detection of RCV-1 in rose in Canada. The sequences of the genome fragments of RCV-1 isolate GO are deposited in GenBank under accession numbers KM598758 (dsRNA 1), KM598759 (dsRNA 2), and KM598760 (dsRNA 3).References:(1) Martin, R. R., and Tzanetakis, I. E. 2008. Plant Dis. 92:1706. https://doi.org/10.1094/PDIS-92-12-1706B Link, ISI, Google Scholar(2) Nibert, M. L., et al. 2014. Virus Res. 188:128. https://doi.org/10.1016/j.virusres.2014.04.007 Crossref, ISI, Google Scholar(3) Sabanadzovic, S., and Abou Ghanem-Sabanadzovic, N. 2008. J. Plant Pathol. 90:287. ISI, Google Scholar(4) Salem, N. M., et al. 2008. Arch. Virol. 153:455. https://doi.org/10.1007/s00705-007-0008-3 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 4 April 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 17 Apr 2015Published: 1 Apr 2015First Look: 25 Nov 2014Accepted: 19 Nov 2014 Pages: 558-558 Information© 2015 The American Phytopathological SocietyCited byPrevalence and molecular characterization of Turkish isolates of the rose virusesCrop Protection, Vol. 143Facing Rose rosette virus : A risk to European rose cultivation3 September 2020 | Plant Pathology, Vol. 69, No. 9High throughput sequencing and RT-qPCR assay reveal the presence of rose cryptic virus-1 in the United Kingdom6 May 2019 | Journal of Plant Pathology, Vol. 101, No. 4Rosa spp. (Rose)6 June 2020Partial characterization of two new viruses in ash belonging to the families Partitiviridae and CaulimoviridaeActa Horticulturae, No. 1191