Quantitative study of HIV-1 Tat peptide and TAR RNA interaction inhibited by poly(allylamine hydrochloride)
2003; Elsevier BV; Volume: 312; Issue: 2 Linguagem: Inglês
10.1016/j.bbrc.2003.10.134
ISSN1090-2104
AutoresH. Vicky Zhao, Dongsheng Dai, Jinru Li, Yi Chen, Long Jiang,
Tópico(s)RNA Research and Splicing
ResumoThe interaction of poly(allylamine hydrochloride) (PAH) with TAR RNA has been studied by quartz crystal microbalance (QCM) cooperating with capillary electrophoresis (CE). Experimental results showed that PAH had high affinity for TAR RNA. In particular, PAH could disrupt the interaction of Tat peptide with TAR RNA, which is critical for HIV-1 virus replication. The approaches described here indicate that they are powerful for studying the binding processes of Tat peptide-TAR RNA and drug-TAR RNA, having great significance for the design of new drug.
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