Stabilization of lethal and hemolytic activities of box jellyfish (Chironex fleckeri) venom
1989; Elsevier BV; Volume: 27; Issue: 4 Linguagem: Inglês
10.1016/0041-0101(89)90206-7
ISSN1879-3150
AutoresAlfio Comis, R. F. Hartwick, M.E.H. Howden,
Tópico(s)Bee Products Chemical Analysis
ResumoA. Comis, R. F. Hartwick and M. E. H. Howden. Stabilization of lethal and hemolytic activities of box jellyfish (Chironex fleckeri) venom. Toxicon27, 439–447, 1989.—The stability of both the lethal and hemolytic activities of box jellyfish (Chironex fleckeri) tentacle extract was assessed after various extraction procedures. Both activities were higher when no buffers or water were used during the initial extraction. Also, when the extract was first filtered through a Sep-pak C18 cartridge, the residual lethal titre, after incubation for 24 hr at room temperature, was increased 16-fold and hemolysis was increased 2.6-fold. Evidence for proteolytic activity in the extract was also obtained and monitored by size exclusion HPLC.
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