Produção Nacional
Revisado por pares
Rat pinealectomy: a modified direct visual approach
2009; Sociedade Brasileira Para o Desenvolvimento da Pesquisa em Cirurgia; Volume: 24; Issue: 4 Linguagem: Inglês
10.1590/s0102-86502009000400013
ISSN1678-2674
AutoresCarla Cristina Maganhin, Ricardo Santos Simões, Luiz Fernando Portugal Fuchs, Ricardo Martins Oliveira-Filho, Manuel de Jesus Simões, Joaquim Evêncio‐Neto, Edmund Chada Baracat, José Maria Soares,
Tópico(s)Circadian rhythm and melatonin
ResumoTo report a new, direct visual approach for rat pinealectomy.Eighty adult female rats (Rattus norvegicus albinus EPM-1 strain) were weighted and anesthetized intraperitoneally with 15 mg/kg xylazine and 30 mg/kg ketamine. The animal was fastened to a dissection table, an incision was made in the skin and the subcutaneous tissue, bringing the lambda into view. The skullcap was opened with a dental drill, bringing the cerebral hemispheres and the superior sagittal sinus into view. The pineal gland, located under the venous sinus, was removed in a single piece using tweezers. Next, the bone fragment was returned to its place and the surgical layers were sutured.This new technique is easy to be done, avoids bleedings and removes only the pineal gland without damage to the remaining encephalon. In addition it makes possible the achievement of a sham surgery, allowing the pineal gland to remain intact.The proposed technique intends to facilitate studies aiming to better understanding the complexity and importance of the pineal gland on reproductive and other body systems.
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