Methyl farnesoate in the prawn Macrobrachium rosenbergii: Synthesis by the mandibular organ in vitro, and titers in the hemolymph
1991; Elsevier BV; Volume: 99; Issue: 4 Linguagem: Inglês
10.1016/0305-0491(91)90157-9
ISSN0305-0491
AutoresAmir Sagi, Ellen Homola, Hans Laufer,
Tópico(s)Neurobiology and Insect Physiology Research
Resumo1. The mandibular organ of Macrobrachium rosenbergii was identified and cultured in the presence of [methyl-3H]-methionine. 2. Radiolabelled methyl farnesoate was extracted and quantitated from cultured glands and culture media of both males and females: variation in synthesis rates was observed (1558–52,652 DPM/hr per gland) between individual prawns. 3. No significant incorporation of isotope into hexane-extractable material was observed in any other tissue cultured. 4. The concentration of methyl farnesoate in the hemolymph of M. rosenbergii was determined using normal phase HPLC with cis-trans (non-biological isomer) as an internal standard. 5. Hemolymph from males contained 17.3 ± 9.5 ng/ml methyl farnesoate. 6. Female hemolymph contained 24 ± 13.1 ng/ml methyl farnesoate.
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